NDLI: CCR7 is mainly expressed in teleost gills, where it defines an IgD+IgM- B lymphocyte subset.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bromage, Erin González Granja, Aitor Luque, Alfonso Castro, Rosario Abós, Beatriz Pignatelli, Jaime Tafalla, Carolina
Description	Country affiliation: Spain Author Affiliation: Castro R ( Centro de Investigación en Sanidad Animal, Instituto Nacional de Investigación Agraria y Alimentaria, Valdeolmos, Madrid 28130, Spain.)
Abstract	Chemokine receptor CCR7, the receptor for both CCL19 and CCL21 chemokines, regulates the recruitment and clustering of circulating leukocytes to secondary lymphoid tissues, such as lymph nodes and Peyer's patches. Even though teleost fish do not have either of these secondary lymphoid structures, we have recently reported a homolog to CCR7 in rainbow trout (Oncorhynchus mykiss). In the present work, we have studied the distribution of leukocytes bearing extracellular CCR7 in naive adult tissues by flow cytometry, observing that among the different leukocyte populations, the highest numbers of cells with membrane (mem)CCR7 were recorded in the gill (7.5 ± 2% CCR7(+) cells). In comparison, head kidney, spleen, thymus, intestine, and peripheral blood possessed <5% CCR7(+) cells. When CCR7 was studied at early developmental stages, we detected a progressive increase in gene expression and protein CCR7 levels in the gills throughout development. Surprisingly, the majority of the CCR7(+) cells in the gills were not myeloid cells and did not express membrane CD8, IgM, nor IgT, but expressed IgD on the cell surface. In fact, most IgD(+) cells in the gills expressed CCR7. Intriguingly, the IgD(+)CCR7(+) population did not coexpress memIgM. Finally, when trout were bath challenged with viral hemorrhagic septicemia virus, the number of CCR7(+) cells significantly decreased in the gills while significantly increased in head kidney. These results provide evidence of the presence of a novel memIgD(+)memIgM(-) B lymphocyte subset in trout that expresses memCCR7 and responds to viral infections. Similarities with IgD(+)IgM(-) subsets in mammals are discussed.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	3
Volume Number	192
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2014-02-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	B-lymphocyte Subsets Metabolism Gene Expression Regulation, Developmental Gills Immunoglobulin D Oncorhynchus Mykiss Receptors, Ccr7 Biosynthesis Animals Antibody Specificity Cytology Growth & Development Head Kidney Hemorrhagic Septicemia, Viral Immunology Immunoglobulin M Lymphoid Tissue Novirhabdovirus Physiology Genetics Organ Specificity Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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