NDLI: Humoral immunity and CD4+ Th1 cells are both necessary for a fully protective immune response upon secondary infection with Brucella melitensis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Letesson, Jean-Jacques Vitry, Marie-Alice Ryffel, Bernhard Muraille, Eric Hanot Mambres, Delphine De Trez, Carl Akira, Shizuo
Description	Author Affiliation: Vitry MA ( Unité de Recherche en Biologie des Microorganismes, Laboratoire d'Immunologie et de Microbiologie, Institut Namurois des Sciences de la Vie, Université de Namur, 5000 Namur, Belgium)
Abstract	Brucella spp are intracellular bacteria that cause brucellosis, one of the most common zoonoses in the world. Given the serious medical consequences of this disease, a safe and effective human vaccine is urgently needed. Efforts to develop this vaccine have been hampered by our lack of understanding of what constitutes a protective memory response against Brucella. In this study, we characterize the cells and signaling pathways implicated in the generation of a protective immune memory response following priming by the injection of heat-killed or live Brucella melitensis 16M. Using a panel of gene-deficient mice, we demonstrated that during a secondary recall response, both the Brucella-specific humoral response and CD4+ Th1 cells must act together to confer protective immunity in the spleen to B. melitensis infection. Humoral protective immunity is induced by the inoculation of both heat-killed and live bacteria, and its development does not require T cells, MyD88/IL-12p35 signaling pathways, or an activation-induced deaminase-mediated isotype switch. In striking contrast, the presence of memory IFN-Î³-producing CD4+ Th1 cells requires the administration of live bacteria and functional MyD88/IL-12p35 pathways. In summary, our work identifies several immune markers closely associated with protective immune memory and could help to define a rational strategy to obtain an effective human vaccine against brucellosis.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	8
Volume Number	192
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2014-04-15
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Brucella Melitensis Immunology Brucellosis Immunity, Humoral Th1 Cells Animals Antibodies, Bacterial Antibody Specificity Bacteremia Prevention & Control Brucella Vaccine Administration & Dosage Metabolism Cd4-positive T-lymphocytes H-2 Antigens Immunologic Memory Interferon-gamma Biosynthesis Interleukin-12 Mice Mice, Knockout Myeloid Differentiation Factor 88 Phenotype Signal Transduction Spleen Cytology Microbiology Vaccines, Inactivated Vaccines, Live, Unattenuated Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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