NDLI: IL-2 phosphorylates STAT5 to drive IFN-Î³ production and activation of human dendritic cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Meley, Daniel Lebranchu, Yvon Heraud, Audrey Herr, Florence Gouilleux, Fabrice Kazma, Ihab Velge-Roussel, Florence Baron, Christophe Lemoine, Roxane
Description	Author Affiliation: Herr F ( L'Équipe d'Accueil 4245, Université François Rabelais, 37032 Tours, France); Lemoine R ( L'Équipe d'Accueil 4245, Université François Rabelais, 37032 Tours, France); Gouilleux F ( Unité Mixte de Recherche 7292, Centre National de la Recherche Scientifique, 37032 Tours, France); Meley D ( L'Équipe d'Accueil 4245, Université François Rabelais, 37032 Tours, France); Kazma I ( L'Équipe d'Accueil 4245, Université François Rabelais, 37032 Tours, France); Heraud A ( L'Équipe d'Accueil 4245, Université François Rabelais, 37032 Tours, France); Velge-Roussel F ( L'Équipe d'Accueil 4245, Université François Rabelais, 37032 Tours, France); Baron C ( L'Équipe d'Accueil 4245, Université François Rabelais, 37032 Tours, France); Lebranchu Y ( L'Équipe d'Accueil 4245, Université François Rabelais, 37032 Tours, France)
Abstract	Human dendritic cells (hDCs) produce IL-2 and express IL-2R -chain (CD25), but the role of IL-2 in DC functions is not well defined. A recent study suggested that the main function of CD25 on hDCs was to transpresent IL-2 to activate T lymphocytes. Our results demonstrate the expression of the three chains of the IL-2R on hDCs and that IL-2 induces STAT5 phosphorylation. Interestingly, use of inhibitors of p-STAT5 revealed that IL-2 increases LPS-induced IFN-Î³ through STAT5 phosphorylation. Finally, we report that IL-2 increases the ability of hDCs to activate helpless CD8(+) T cells, most likely because of IL-2-triggered IFN-Î³ synthesis, as we previously described. For the first time, to our knowledge, we disclose that IL-2 induces monocyte-derived hDC's functional maturation and activation through IL-2R binding. Interestingly, our study suggests a direct effect of anti-CD25 mAbs on hDCs that may contribute to their clinical efficacy.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	12
Volume Number	192
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2014-06-15
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Dendritic Cells Immunology Interferon-gamma Interleukin-2 Stat5 Transcription Factor Antibodies Pharmacology Cytology Interleukin-2 Receptor Alpha Subunit Antagonists & Inhibitors Lipopolysaccharides Monocytes Phosphorylation Drug Effects Clinical Trial Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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