NDLI: Dectin-1 pathway activates robust autophagy-dependent unconventional protein secretion in human macrophages.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Öhman, Tiina Veckman, Ville Wolff, Henrik Saijo, Shinobu Teirilä, Laura Lahesmaa-Korpinen, Anna-Maria Hautaniemi, Sampsa Matikainen, Sampsa Nyman, Tuula A. Cypryk, Wojciech
Description	Author Affiliation: Öhman T ( Institute of Biotechnology, University of Helsinki, 00014 Helsinki, Finland); Teirilä L ( Finnish Institute of Occupational Health, 00250 Helsinki, Finland); Lahesmaa-Korpinen AM ( Computational Systems Biology Laboratory, Institute of Biomedicine, Genome-Scale Biology Research Program, Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland); Cypryk W ( Institute of Biotechnology, University of Helsinki, 00014 Helsinki, Finland); Veckman V ( Finnish Institute of Occupational Health, 00250 Helsinki, Finland); Saijo S ( Department of Molecular Immunology, Medical Mycology Research Center, Chiba University, Chiba 260-8673, Japan); Wolff H ( Finnish Institute of Occupational Health, 00250 Helsinki, Finland); Hautaniemi S ( Computational Systems Biology Laboratory, Institute of Biomedicine, Genome-Scale Biology Research Program, Faculty of Medicine, University of Helsinki, 00014 Helsinki, Finland); Nyman TA ( Institute of Biotechnology, University of Helsinki, 00014 Helsinki, Finland); Matikainen S ( Finnish Institute of Occupational Health, 00250 Helsinki, Finland)
Abstract	Dectin-1 is a membrane-bound pattern recognition receptor for ß-glucans, which are the main constituents of fungal cell walls. Detection of ß-glucans by dectin-1 triggers an effective innate immune response. In this study, we have used a systems biology approach to provide the first comprehensive characterization of the secretome and associated intracellular signaling pathways involved in activation of dectin-1/Syk in human macrophages. Transcriptome and secretome analysis revealed that the dectin-1 pathway induced significant gene expression changes and robust protein secretion in macrophages. The enhanced protein secretion correlated only partly with increased gene expression. Bioinformatics combined with functional studies revealed that the dectin-1/Syk pathway activates both conventional and unconventional, vesicle-mediated, protein secretion. The unconventional protein secretion triggered by the dectin-1 pathway is dependent on inflammasome activity and an active autophagic process. In conclusion, our results reveal that unconventional protein secretion has an important role in the innate immune response against fungal infections.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	12
Volume Number	192
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2014-06-15
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Autophagy Immunology Gene Expression Regulation Immunity, Innate Inflammasomes Lectins, C-type Macrophages Secretion Intracellular Signaling Peptides And Proteins Metabolism Mycoses Protein-tyrosine Kinases Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in