NDLI: Cutting Edge: Regulation of Exosome Secretion by the Integral MAL Protein in T Cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Fernández-Martín, Laura Martínez-Alonso, Emma Alonso, Miguel A. Martínez-Menárguez, José Angel Ventimiglia, Leandro N. Andrés, Germán Antón, Olga M. Guerra, Milagros
Description	Author Affiliation: Ventimiglia LN ( Departamento de Biología Celular e Inmunología, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain); Fernández-Martín L ( Departamento de Biología Celular e Inmunología, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain); Martínez-Alonso E ( Departamento de Biología Celular e Histología, Facultad de Medicina, Instituto Murciano de Investigación Biosanitaria, Universidad de Murcia, 30100 Murcia, Spain); Antón OM ( Departamento de Biología Celular e Inmunología, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain); Guerra M ( Unidad de Microscopía Electrónica, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.); Martínez-Menárguez JA ( Departamento de Biología Celular e Histología, Facultad de Medicina, Instituto Murciano de Investigación Biosanitaria, Universidad de Murcia, 30100 Murcia, Spain); Andrés G ( Unidad de Microscopía Electrónica, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain.); Alonso MA ( Departamento de Biología Celular e Inmunología, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Cantoblanco, 28049 Madrid, Spain)
Abstract	Exosomes secreted by T cells play an important role in coordinating the immune response. HIV-1 Nef hijacks the route of exosome secretion of T cells to modulate the functioning of uninfected cells. Despite the importance of the process, the protein machinery involved in exosome biogenesis is yet to be identified. In this study, we show that MAL, a tetraspanning membrane protein expressed in human T cells, is present in endosomes that travel toward the plasma membrane for exosome secretion. In the absence of MAL, the release of exosome particles and markers was greatly impaired. This effect was accompanied by protein sorting defects at multivesicular endosomes that divert the exosomal marker CD63 to autophagic vacuoles. Exosome release induced by HIV-1 Nef was also dependent on MAL expression. Therefore, MAL is a critical element of the machinery for exosome secretion and may constitute a target for modulating exosome secretion by human T cells.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	3
Volume Number	195
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2015-08-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Hiv Infections Immunology Hiv-1 Multivesicular Bodies Secretion Myelin And Lymphocyte-associated Proteolipid Proteins Metabolism T-lymphocytes Nef Gene Products, Human Immunodeficiency Virus Antigens, Cd63 Cell Line, Tumor Cell Membrane Jurkat Cells Genetics Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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