NDLI: Programming of Influenza Vaccine Broadness and Persistence by Mucoadhesive Polymer-Based Adjuvant Systems.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lee, Jong-Soo Chowdhury, Mohammed Y. E. Lim, Yong Taik Noh, Hyun Jong Cho, Seonghun Poo, Haryoung Sung, Moon-Hee Park, Hye Sun Kim, Chul-Joong Kim, Jae-Hoon
Description	Author Affiliation: Noh HJ ( Department of Chemical Engineering, Sungkyunkwan University Advanced Institute of Nanotechnology, Suwon 440-746, South Korea); Chowdhury MY ( College of Veterinary Medicine (BK21 Plus Program), Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 305-764, South Korea); Cho S ( College of Veterinary Medicine (BK21 Plus Program), Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 305-764, South Korea); Kim JH ( College of Veterinary Medicine (BK21 Plus Program), Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 305-764, South Korea); Park HS ( Korea Basic Science Institute, Chungbuk 363-883, South Korea); Kim CJ ( College of Veterinary Medicine (BK21 Plus Program), Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 305-764, South Korea); Poo H ( Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, South Korea); Sung MH ( Department of Advanced Fermentation Fusion Science and Technology, Kookmin University, Seoul 136-702, South Korea.); Lee JS ( College of Veterinary Medicine (BK21 Plus Program), Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon 305-764, South Korea); Lim YT ( Department of Chemical Engineering, Sungkyunkwan University Advanced Institute of Nanotechnology, Suwon 440-746, South Korea)
Abstract	The development of an anti-influenza vaccine with the potential for cross-protection against seasonal drift variants as well as occasionally emerging reassortant viruses is essential. In this study, we successfully generated a novel anti-influenza vaccine system combining conserved matrix protein 2 (sM2) and stalk domain of hemagglutinin (HA2) fusion protein (sM2HA2) and poly-Î³-glutamic acid (Î³-PGA)-based vaccine adjuvant systems that can act as a mucoadhesive delivery vehicle of sM2HA2 as well as a robust strategy for the incorporation of hydrophobic immunostimulatory 3-O-desacyl-4'-monophosphoryl lipid A (MPL) and QS21. Intranasal coadministration of sM2HA2 and the combination adjuvant Î³-PGA/MPL/QS21 (CA-PMQ) was able to induce a high degree of protective mucosal, systemic, and cell-mediated immune responses. The sM2HA2/CA-PMQ immunization was able to prevent disease symptoms, confering complete protection against lethal infection with divergent influenza subtypes (H5N1, H1N1, H5N2, H7N3, and H9N2) that lasted for at least 6 mo. Therefore, our data suggest that mucosal administration of sM2HA2 in combination with CA-PMQ could be a potent strategy for a broad cross-protective influenza vaccine, and CA-PMQ as a mucosal adjuvant could be used for effective mucosal vaccines.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	5
Volume Number	195
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2015-09-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Adjuvants, Immunologic Chemistry Influenza Vaccines Immunology Polymers Administration & Dosage Animals Cross Protection Hemagglutinin Glycoproteins, Influenza Virus Host-pathogen Interactions Immune System Immunity, Cellular Immunity, Mucosal Immunization Influenza A Virus, H1n1 Subtype Physiology Influenza A Virus, H5n1 Subtype Influenza A Virus, H5n2 Subtype Influenza A Virus, H7n3 Subtype Influenza A Virus, H9n2 Subtype Mice, Inbred Balb C Orthomyxoviridae Infections Prevention & Control Virology Polyglutamic Acid Analogs & Derivatives Viral Matrix Proteins Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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