| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Claas, Frans H. J. Blokhuis, Jeroen H. Parham, Peter de Groot, Natasja G. Guethlein, Lisbeth A. Mulder, Arend Doxiadis, Gaby G. M. Bontrop, Ronald E. Van Der Wiel, Marit K. H. Heijmans, Corrine M. C. |
| Description |
Author Affiliation: de Groot NG ( Comparative Genetics and Refinement, Biomedical Primate Research Centre, 2288 GJ Rijswijk, the Netherlands); Heijmans CM ( Comparative Genetics and Refinement, Biomedical Primate Research Centre, 2288 GJ Rijswijk, the Netherlands); van der Wiel MK ( Comparative Genetics and Refinement, Biomedical Primate Research Centre, 2288 GJ Rijswijk, the Netherlands); Blokhuis JH ( Comparative Genetics and Refinement, Biomedical Primate Research Centre, 2288 GJ Rijswijk, the Netherlands); Mulder A ( Immunohaematology and Blood Transfusion, Leiden University Medical Center, 2333 ZA Leiden, the Netherlands); Guethlein LA ( Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305); Doxiadis GG ( Comparative Genetics and Refinement, Biomedical Primate Research Centre, 2288 GJ Rijswijk, the Netherlands); Claas FH ( Immunohaematology and Blood Transfusion, Leiden University Medical Center, 2333 ZA Leiden, the Netherlands); Parham P ( Department of Structural Biology, Stanford University School of Medicine, Stanford, CA 94305); Bontrop RE ( Comparative Genetics and Refinement, Biomedical Primate Research Centre, 2288 GJ Rijswijk, the Netherlands) |
| Abstract |
MHC haplotypes of humans and the African great ape species have one copy of the MHC-A, -B, and -C genes. In contrast, MHC haplotypes of orangutans, the Asian great ape species, exhibit variation in the number of gene copies. An in-depth analysis of the MHC class I gene repertoire in the two orangutan species, Pongo abelii and Pongo pygmaeus, is presented in this article. This analysis involved Sanger and next-generation sequencing methodologies, revealing diverse and complicated transcription profiles for orangutan MHC-A, -B, and -C. Thirty-five previously unreported MHC class I alleles are described. The data demonstrate that each orangutan MHC haplotype has one copy of the MHC-A gene, and that the MHC-B region has been subject to duplication, giving rise to at least three MHC-B genes. The MHC-B*03 and -B*08 lineages of alleles each account for a separate MHC-B gene. All MHC-B*08 allotypes have the C1-epitope motif recognized by killer cell Ig-like receptor. At least one other MHC-B gene is present, pointing to MHC-B alleles that are not B*03 or B*08. The MHC-C gene is present only on some haplotypes, and each MHC-C allotype has the C1-epitope. The transcription profiles demonstrate that MHC-A alleles are highly transcribed, whereas MHC-C alleles, when present, are transcribed at very low levels. The MHC-B alleles are transcribed to a variable extent and over a wide range. For those orangutan MHC class I allotypes that are detected by human monoclonal anti-HLA class I Abs, the level of cell-surface expression of proteins correlates with the level of transcription of the allele. |
| ISSN |
00221767 |
| e-ISSN |
15506606 |
| Journal |
The Journal of Immunology |
| Issue Number |
2 |
| Volume Number |
196 |
| Language |
English |
| Publisher |
The American Association of Immunologists |
| Publisher Date |
2016-01-15 |
| Publisher Place |
United States |
| Access Restriction |
Open |
| Subject Keyword |
Genes, Mhc Class I Pongo Abelii Genetics Pongo Pygmaeus Transcriptome Amino Acid Sequence Animals Evolution, Molecular Haplotypes High-throughput Nucleotide Sequencing Phylogeny Reverse Transcriptase Polymerase Chain Reaction Research Support, Non-u.s. Gov't Discipline Immunology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Immunology and Allergy Immunology |
