NDLI: TRPV4-Mediated Detection of Hyposmotic Stress by Skin Keratinocytes Activates Developmental Immunity.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Pardo-Pastor, Carlos Rubio-Moscardo, Fanny Mulero, Victoriano Liarte, Sergio de Oliveira, Sofia Montalban-Arques, Ana Galindo-Villegas, Jorge Meseguer, José Valverde, Miguel A.
Description	Author Affiliation: Galindo-Villegas J ( Department of Cell Biology and Histology, Faculty of Biology, Institute of Biomedical Research of Murcia-Arrixaca, Espinardo Campus, University of Murcia, 30100 Murcia, Spain); Montalban-Arques A ( Department of Cell Biology and Histology, Faculty of Biology, Institute of Biomedical Research of Murcia-Arrixaca, Espinardo Campus, University of Murcia, 30100 Murcia, Spain); Liarte S ( Department of Cell Biology and Histology, Faculty of Biology, Institute of Biomedical Research of Murcia-Arrixaca, Espinardo Campus, University of Murcia, 30100 Murcia, Spain); de Oliveira S ( Department of Cell Biology and Histology, Faculty of Biology, Institute of Biomedical Research of Murcia-Arrixaca, Espinardo Campus, University of Murcia, 30100 Murcia, Spain); Pardo-Pastor C ( Laboratory of Molecular Physiology and Channelopathies, Department of Experimental and Health Sciences, Pompeu Fabra University, 08002 Barcelona, Spain.); Rubio-Moscardo F ( Laboratory of Molecular Physiology and Channelopathies, Department of Experimental and Health Sciences, Pompeu Fabra University, 08002 Barcelona, Spain.); Meseguer J ( Department of Cell Biology and Histology, Faculty of Biology, Institute of Biomedical Research of Murcia-Arrixaca, Espinardo Campus, University of Murcia, 30100 Murcia, Spain); Valverde MA ( Laboratory of Molecular Physiology and Channelopathies, Department of Experimental and Health Sciences, Pompeu Fabra University, 08002 Barcelona, Spain.); Mulero V ( Department of Cell Biology and Histology, Faculty of Biology, Institute of Biomedical Research of Murcia-Arrixaca, Espinardo Campus, University of Murcia, 30100 Murcia, Spain)
Abstract	As an organism is exposed to pathogens during very early development, specific defense mechanisms must take effect. In this study, we used a germ-free zebrafish embryo model to show that osmotic stress regulates the activation of immunity and host protection in newly hatched embryos. Mechanistically, skin keratinocytes were responsible for both sensing the hyposmolarity of the aquatic environment and mediating immune effector mechanisms. This occurred through a transient potential receptor vanilloid 4/Ca(2+)/TGF-ß-activated kinase 1/NF-κB signaling pathway. Surprisingly, the genes encoding antimicrobial effectors, which do not have the potential to cause tissue damage, are constitutively expressed during development, independently of both commensal microbes and osmotic stress. Our results reveal that osmotic stress is associated with the induction of developmental immunity in the absence of tissue damage and point out to the embryo skin as the first organ with full capacities to mount an innate immune response.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	2
Volume Number	196
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2016-01-15
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Immunity, Innate Immunology Keratinocytes Skin Embryology Trpv Cation Channels Zebrafish Proteins Zebrafish Animals Embryo, Nonmammalian Fluorescent Antibody Technique Osmotic Pressure Reverse Transcriptase Polymerase Chain Reaction Transcriptome Transfection Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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