NDLI: A high-fat diet elicits differential responses in genes coordinating oxidative metabolism in skeletal muscle of lean and obese individuals.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Gavin, T. P. Koves, T. R. Consitt, L. A. Muoio, D. M. Boyle, K. E. Houmard, J. A. Holbert, D. Neufer, P. D. Canham, J. P. Zheng, D. Ilkayeva, O.
Description	Country affiliation: United States Author Affiliation: Boyle KE ( Human Performance Laboratory, East Carolina University, Greenville, North Carolina 27858, USA. kristen.boyle@ucdenver.edu)
Abstract	CONTEXT: In lean individuals, increasing dietary lipid can elicit an increase in whole body lipid oxidation; however, with obesity the capacity to respond to changes in substrate availability appears to be compromised. OBJECTIVE: To determine whether the responses of genes regulating lipid oxidation in skeletal muscle differed between lean and insulin resistant obese humans upon exposure to a high-fat diet (HFD). DESIGN AND SETTING: A 5-d prospective study conducted in the research unit of an academic center. PARTICIPANTS: Healthy, lean (n = 12; body mass index = 22.1 ± 0.6 kg/m(2)), and obese (n=10; body mass index = 39.6 ± 1.7 kg/m(2)) males and females, between ages 18 and 30. INTERVENTION: Participants were studied before and after a 5-d HFD (65% fat). MAIN OUTCOME MEASURES: Skeletal muscle biopsies (vastus lateralis) were obtained in the fasted and fed states before and after the HFD and mRNA content for genes involved with lipid oxidation determined. Skeletal muscle acylcarnitine content was determined in the fed states before and after the HFD. RESULTS: Peroxisome proliferator activated receptor (PPAR) mRNA content increased in lean, but not obese, subjects after a single high-fat meal. From Pre- to Post-HFD, mRNA content exhibited a body size × HFD interaction, where the lean individuals increased while the obese individuals decreased mRNA content for pyruvate dehydrogenase kinase 4, uncoupling protein 3, PPAR , and PPARÎ³ coactivator-1 (P ≤ 0.05). In the obese subjects medium-chain acylcarnitine species tended to accumulate, whereas no change or a reduction was evident in the lean individuals. CONCLUSIONS: These findings indicate a differential response to a lipid stimulus in the skeletal muscle of lean and insulin resistant obese humans.
ISSN	0021972X
e-ISSN	19457197
DOI	10.1210/jc.2010-2253
Journal	The Journal of Clinical Endocrinology & Metabolism
Issue Number	3
Volume Number	96
Language	English
Publisher	Oxford University Press
Publisher Date	2011-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Dietary Fats Pharmacology Lipid Metabolism Genetics Muscle, Skeletal Metabolism Obesity Adolescent Blood Glucose Body Mass Index Carnitine Analogs & Derivatives Diet Fatty Acids, Nonesterified Blood Gene Expression Regulation Drug Effects Insulin Ion Channels Mitochondrial Proteins Chemistry Oxidation-reduction Ppar Alpha Biosynthesis Pyruvate Dehydrogenase (lipoamide) Rna, Messenger Spectrometry, Mass, Electrospray Ionization Research Support, N.i.h., Extramural Discipline Endocrinology Discipline Metabolism
Content Type	Text
Resource Type	Article
Subject	Biochemistry (medical) Endocrinology, Diabetes and Metabolism Clinical Biochemistry Biochemistry Endocrinology

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