NDLI: Sustained Endoplasmic Reticulum Stress as a Cofactor of Oxidative Stress in Decidual Cells from Patients with Early Pregnancy Loss

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sheng, Jian-Zhong Yin, Li-Jun Zhang, Yu Leung, Peter C. K. Huang, He-Feng He, Wei-Hua Liu, Ai-Xia Lv, Ping-Ping
Description	Country affiliation: China Author Affiliation: Liu AX ( Department of Reproductive Endocrinology, Women's Hospital, School of Medicine, Zhejiang University, 1 Xueshi Road, Hangzhou, Zhejiang 310006, China.)
Abstract	BACKGROUND: Oxidative stress is a common pathological background for different etiologies of early pregnancy loss (EPL). It has been suggested that elevated reactive oxygen species trigger endoplasmic reticulum (ER) stress by influencing ER function. However, it is unclear whether ER stress is associated with EPL. OBJECTIVES: The aim of the study was to determine whether and how ER stress occurs during the development of EPL. APPROACHES: Proteomic analysis was performed on decidua from women with EPL, and then ER stress markers, redox status, apoptotic features, and cell viability were analyzed in EPL decidual cells (DCs). RESULTS: EPL decidua were characterized by decreased levels of glucose-regulated protein 78 (GPR78) and valosin-containing protein and burdened with ubiquitinated proteins. Evidence of ER stress-induced apoptosis in EPL DCs was demonstrated by extensive dilation of ER, morphological features of apoptosis, and activation of caspase-4 and caspase-12. Furthermore, H(2)O(2) reduced the viabilities in both EPL and control DCs, whereas EPL DCs were more vulnerable to additional OS challenge than the controls as a result of failed induction of GRP78 expression. The cell survival percentages of DCs were dose-dependently reduced by H(2)O(2) and could be reversed in the presence of vitamin E. This effect was partly mediated by reducing the amount of misfolded proteins rather than regulating GRP78 expression. CONCLUSIONS: The sum of these observations demonstrate for the first time that sustained ER stress occurs in EPL DCs and the potentially vicious relationship between ER stress and oxidative stress is likely to play an important role in the development of EPL.
ISSN	0021972X
e-ISSN	19457197
Journal	The Journal of Clinical Endocrinology & Metabolism
Issue Number	3
Volume Number	96
Language	English
Publisher	Oxford University Press
Publisher Date	2011-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Abortion, Spontaneous Metabolism Pathology Decidua Cytology Endoplasmic Reticulum Oxidative Stress Physiology Genetics Adenosine Triphosphatases Blotting, Western Caspases Cell Cycle Proteins Cell Separation Cell Survival Dose-response Relationship, Drug Heat-shock Proteins Hydrogen Peroxide Pharmacology Immunohistochemistry Microscopy, Electron, Transmission Pregnancy Proteomics Reactive Oxygen Species Research Support, Non-u.s. Gov't Discipline Endocrinology Discipline Metabolism
Content Type	Text
Resource Type	Article
Subject	Biochemistry (medical) Endocrinology, Diabetes and Metabolism Clinical Biochemistry Biochemistry Endocrinology

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