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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Fa, Huanbao Huo, Danqun Luo, Xiaogang Yang, Limin Yang, Mei Hou, Changjun |
| Description | Author Affiliation: Yang L ( College of Bioengineering/Key Laboratory of Biorheology Science and Technology of Ministry of Education, Chongqing University, Chongqing, PR China.) |
| Abstract | The interaction of monosulfonate tetraphenyl porphyrin (H(2)TPPS(1)) with plant-esterase was investigated using fluorescence and UV-vis absorption spectroscopy. Fluorescence quenching, from which the binding parameters were evaluated, revealed that the quenching of the esterase by H(2)TPPS(1) resulted from the formation of a dye-esterase complex. According to the modified Stern-Volmer equation, the effective quenching constants (K(a)) between H(2)TPPS(1) and plant-esterase at four different temperatures (297 K, 300 K, 303 K, and 306 K) were obtained to be 14.132×10(5), 5.734×10(5), 2.907×10(5), and 2.291×10(5) M(-1), respectively. The thermodynamic parameters, enthalpy change (ΔH) and entropy change (ΔS) for the reaction were calculated to be -181.67 kJ M(-1) and -0.49 kJ M(-1)K(-1), indicating that van der Waals force and hydrogen bonds were the dominant intermolecular force in stabilizing the complex. Site marker competitive experiments showed that the binding of H(2)TPPS(1) to plant-esterase primarily took place in the active site. The binding distance (r) was obtained to be 5.99 nm according to Förster theory of non-radioactive energy transfer. The conformation of plant-esterase was investigated by synchronous fluorescence and UV-vis absorption spectroscopy, and the results confirmed some micro-environmental and conformational changes of plant-esterase molecules. |
| ISSN | 13861425 |
| Issue Number | 5 |
| Volume Number | 78 |
| e-ISSN | 18733557 |
| Journal | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-05-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Esterases Metabolism Plants Enzymology Porphyrins Spectrometry, Fluorescence Methods Binding Sites Binding, Competitive Energy Transfer Chemistry Fluorescence Hydrogen-ion Concentration Kinetics Protein Binding Protein Conformation Spectrophotometry, Ultraviolet Temperature Journal Article Research Support, Non-u.s. Gov't Discipline Spectroscopy |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Atomic and Molecular Physics, and Optics Analytical Chemistry Instrumentation |
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