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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Atta, Diaa Okasha, Ali |
| Description | Author Affiliation: Atta D ( Spectroscopy Department, Physics Division, National Research Center, 12311 Dokki, Cairo, Egypt. Electronic address: dk.yousef@nrc.sci.eg.); Okasha A ( Spectroscopy Department, Physics Division, National Research Center, 12311 Dokki, Cairo, Egypt.) |
| Abstract | In this article, we discussed some single molecule spectroscopy techniques and methods. We have chosen the simplicity in this survey based on our laboratory experience in this field. We concentrated on the imaging by both techniques the wide field and the scanning microscopes. Other imaging enhancements on the technique like extended resolution wide field, the total internal reflection imaging, and its derivatives are also reviewed. In addition to the imaging techniques, some diffusion techniques also are discussed like fluorescence correlation spectroscopy. The related methods like Forester resonance transfer, photo-induced electron transfer and anisotropy (steady state and time decay) are also discussed. In addition, we elucidated some simple details about the theory behind the FCS and its resulting curve fitting. This review is preceded by general introduction and ended with the conclusion. |
| ISSN | 13861425 |
| Volume Number | 135 |
| e-ISSN | 18733557 |
| Journal | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-01-25 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Lasers Spectrum Analysis Methods Electrons Fluorescence Resonance Energy Transfer Imaging, Three-dimensional Light Journal Article Research Support, Non-u.s. Gov't Review Discipline Spectroscopy |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Atomic and Molecular Physics, and Optics Analytical Chemistry Instrumentation |
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