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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Shi, Huimin Feng, Suling Gong, Yijun Cui, Yi |
| Description | Country affiliation: China Author Affiliation: Shi H ( School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China); Cui Y ( School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China.); Gong Y ( School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China.); Feng S ( School of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China. Electronic address: slfeng@htu.cn.) |
| Abstract | A fluorescent probe has been developed for the determination of guanine based on the quenched fluorescence signal of Cu(2+)/eosin Y. Cu(2+) interacted with eosin Y, resulting in fluorescence quenching. Subsequently, with the addition of guanine to the Cu(2+)/eosin Y system, guanine reacted with Cu(2+) to form 1:1 chelate cation, which further combined with eosin Y to form a 1:1 ternary ion-association complex by electrostatic attraction and hydrophobic interaction, resulting in significant decrease of the fluorescence. Hence, a fluorescent system was constructed for rapid, sensitive and selective detection of guanine with a detection limit as low as 1.5 nmol L(-1) and a linear range of 3.3-116 nmol L(-1). The method has been applied satisfactorily to the determination of guanine in DNA and urine samples with the recoveries from 98.7% to 105%. This study significantly expands the realm of application of ternary ion-association complex in fluorescence probe. |
| ISSN | 13861425 |
| Journal | Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy |
| Volume Number | 161 |
| e-ISSN | 18733557 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-05-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Spectroscopy |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Atomic and Molecular Physics, and Optics Analytical Chemistry Instrumentation |
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