NDLI: A device for rapid and quantitative measurement of cardiac myocyte contractility

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A device for rapid and quantitative measurement of cardiac myocyte contractility

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A device for rapid and quantitative measurement of cardiac myocyte contractility

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Malhotra, Ricky Li, Tao Gaitas, Angelo Herron, Todd Jalife, José
Description	Country affiliation: United States Author Affiliation: Gaitas A ( Kytaro, Inc., 11200 SW 8th Street, MARC 430, Miami, Florida 33199, USA.); Malhotra R ( Kytaro, Inc., 11200 SW 8th Street, MARC 430, Miami, Florida 33199, USA.); Li T ( Electrical Engineering and Computer Science, University of Michigan, 1301 Beal Ave., Ann Arbor, Michigan 48109, USA.); Herron T ( Center for Arrhythmia Research, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan 48109, USA.); Jalife J ( Center for Arrhythmia Research, Department of Internal Medicine, University of Michigan, Ann Arbor, Michigan 48109, USA.)
Abstract	Cardiac contractility is the hallmark of cardiac function and is a predictor of healthy or diseased cardiac muscle. Despite advancements over the last two decades, the techniques and tools available to cardiovascular scientists are limited in their utility to accurately and reliably measure the amplitude and frequency of cardiomyocyte contractions. Isometric force measurements in the past have entailed cumbersome attachment of isolated and permeabilized cardiomyocytes to a force transducer followed by measurements of sarcomere lengths under conditions of submaximal and maximal Ca(2+) activation. These techniques have the inherent disadvantages of being labor intensive and costly. We have engineered a micro-machined cantilever sensor with an embedded deflection-sensing element that, in preliminary experiments, has demonstrated to reliably measure cardiac cell contractions in real-time. Here, we describe this new bioengineering tool with applicability in the cardiovascular research field to effectively and reliably measure cardiac cell contractility in a quantitative manner. We measured contractility in both primary neonatal rat heart cardiomyocyte monolayers that demonstrated a beat frequency of 3 Hz as well as human embryonic stem cell-derived cardiomyocytes with a contractile frequency of about 1 Hz. We also employed the ß-adrenergic agonist isoproterenol (100 nmol l(-1)) and observed that our cantilever demonstrated high sensitivity in detecting subtle changes in both chronotropic and inotropic responses of monolayers. This report describes the utility of our micro-device in both basic cardiovascular research as well as in small molecule drug discovery to monitor cardiac cell contractions.
ISSN	00346748
e-ISSN	10897623
DOI	10.1063/1.4915500
Journal	Review of Scientific Instruments
Issue Number	3
Volume Number	86
Language	English
Publisher	American Institute of Physics
Publisher Date	2015-03-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Microtechnology Instrumentation Myocardial Contraction Physiology Myocytes, Cardiac Adrenergic Beta-agonists Pharmacology Animals Animals, Newborn Cells, Cultured Equipment Design Human Embryonic Stem Cells Drug Effects Isoproterenol Microscopy, Electron, Scanning Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Discipline Physics Discipline Chemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Instrumentation

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