| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Wubshet, Sileshi G. Jäger, Anna K. Nyberg, Nils T. Jønsson, Louise H. Staerk, Dan Tahtah, Yousof Kongstad, Kenneth T. Qinglei, Sun |
| Description |
Country affiliation: Denmark Author Affiliation: Tahtah Y ( Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark.); Kongstad KT ( Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark.); Wubshet SG ( Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark.); Nyberg NT ( Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark.); Jønsson LH ( Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark.); Jäger AK ( Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark.); Qinglei S ( Shandong Analysis and Test Center, Shandong, People's Republic of China.); Staerk D ( Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, DK-2100 Copenhagen, Denmark. Electronic address: ds@sund.ku.dk.) |
| Abstract |
In this work, development of a new microplate-based high-resolution profiling assay using recombinant human aldose reductase is presented. Used together with high-resolution radical scavenging and high-resolution -glucosidase assays, it provided the first report of a triple aldose reductase/ -glucosidase/radical scavenging high-resolution inhibition profile - allowing proof of concept with Radix Scutellariae crude extract as a polypharmacological herbal drug. The triple bioactivity high-resolution profiles were used to pinpoint bioactive compounds, and subsequent structure elucidation was performed with hyphenated high-performance liquid chromatography-high-resolution mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy. The only -glucosidase inhibitor was baicalein, whereas main aldose reductase inhibitors in the crude extract were baicalein and skullcapflavone II, and main radical scavengers were ganhuangemin, viscidulin III, baicalin, oroxylin A 7-O-glucuronide, wogonoside, baicalein, wogonin, and skullcapflavone II. |
| ISSN |
00219673 |
| Volume Number |
1408 |
| e-ISSN |
18733778 |
| Journal |
Journal of Chromatography A |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2015-08-21 |
| Publisher Place |
Netherlands |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Aldehyde Reductase Metabolism Free Radical Scavengers Analysis Hypoglycemic Agents Scutellaria Baicalensis Chemistry Alpha-glucosidases Chromatography, High Pressure Liquid Glycoside Hydrolase Inhibitors Humans Magnetic Resonance Spectroscopy Methods Plant Extracts Recombinant Proteins Solid Phase Extraction Journal Article Research Support, Non-u.s. Gov't Discipline Analytical Chemistry |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Organic Chemistry Medicine Analytical Chemistry Biochemistry |
