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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kucera, Lukás Pospísil, Tomás Aturki, Zeineb Fanali, Salvatore Bednár, Petr |
| Description | Author Affiliation: Kucera L ( Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. listopadu 12, 779 00 Olomouc, Czech Republic); Fanali S ( Institute of Chemical Methodologies, Italian National Research Council, Area della Ricerca di Roma I, Via Salaria Km 29,300, Monterotondo, 00015 Rome, Italy.); Aturki Z ( Institute of Chemical Methodologies, Italian National Research Council, Area della Ricerca di Roma I, Via Salaria Km 29,300, Monterotondo, 00015 Rome, Italy.); Pospísil T ( Centre of the Region Haná for Biotechnological and Agricultural Research, Department of Chemical Biology and Genetics, Faculty of Science, Palacký University, Slechtitelu 27, 78371 Olomouc, Czech Republic.); Bednár P ( Regional Centre of Advanced Technologies and Materials, Department of Analytical Chemistry, Faculty of Science, Palacký University, 17. listopadu 12, 779 00 Olomouc, Czech Republic. Electronic address: petr.bednar@upol.cz.) |
| Abstract | Nano-liquid chromatography and conventional HPLC were used for the separation of diastereomers of (+)-catechin-ethyl-malvidin-3-glucoside. Those bridged anthocyanin dyes were obtained by reaction of (+)-catechin with malvidin-3-glucoside in the presence of acetaldehyde. Both diastereomers were isolated with semipreparative chromatography and their structures were confirmed by nuclear magnetic resonance and mass spectrometry. In-laboratory prepared capillary columns packed with fully porous particles Chromosphere C18, dp=3µm, core-shell particles Kinetex C18, dp=2.6µm (100µm i.d.) and monolithic column Chromolith CapRod (100µm i.d.) were used for the separation of (+)-catechin, malvidin-3-glucoside and both diastereomers. Chromosphere C18 stationary phase provided the best chromatographic performance. Mobile phase containing water:acetonitrile (80:20) acidified with trifluoroacetic acid (0.1%, v/v/v) was used in an isocratic elution mode with a flow rate of 360nLmin(-1). Separation of studied compounds was achieved in less than 7min under optimized conditions. The nano-liquid chromatographic method and a conventional HPLC one using the same fully porous particles (Chromosphere C18, 3µm, 100mm×4.6mm) were compared providing higher separation efficiency with the first analytical method and similar selectivity. A better peak symmetry and higher resolution of the studied diastereomers was achieved by conventional chromatography. Nevertheless, nano-liquid chromatography appeared to be useful for the separation of complex anthocyanin dyes and can be utilized for their analysis in plant and food micro-samples. The developed method was used for analysis of red wine grape pomace. |
| ISSN | 00219673 |
| Volume Number | 1428 |
| e-ISSN | 18733778 |
| Journal | Journal of Chromatography A |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-01-08 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Chromatography, High Pressure Liquid Food Analysis Methods Acetaldehyde Chemistry Acetonitriles Anthocyanins Catechin Glucosides Mass Spectrometry Porosity Vitis Comparative Study Journal Article Research Support, Non-u.s. Gov't Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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