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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Lazzeri, L. Camborata, C. Pagnotta, E. Spinozzi, S. Franco, P. Ugolini, L. Roda, A. |
| Description | Country affiliation: Italy Author Affiliation: Franco P ( Department of 'Chemistry G. Ciamician', University of Bologna, Via Selmi 2, 40126 Bologna, Italy.); Spinozzi S ( Department of 'Chemistry G. Ciamician', University of Bologna, Via Selmi 2, 40126 Bologna, Italy.); Pagnotta E ( Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di ricerca per le colture industriali (CRA-CIN), Via di Corticella 133, 40128 Bologna, Italy.); Lazzeri L ( Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di ricerca per le colture industriali (CRA-CIN), Via di Corticella 133, 40128 Bologna, Italy.); Ugolini L ( Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di ricerca per le colture industriali (CRA-CIN), Via di Corticella 133, 40128 Bologna, Italy.); Camborata C ( Department of 'Chemistry G. Ciamician', University of Bologna, Via Selmi 2, 40126 Bologna, Italy.); Roda A ( Department of 'Chemistry G. Ciamician', University of Bologna, Via Selmi 2, 40126 Bologna, Italy. Electronic address: aldo.roda@unibo.it.) |
| Abstract | A new high pressure liquid chromatography-electrospray ionization-tandem mass spectrometry method for the simultaneous determination of glucosinolates, as glucoraphanin and glucoerucin, and the corresponding isothiocyanates, as sulforaphane and erucin, was developed and applied to quantify these compounds in Eruca sativa defatted seed meals and enriched functional foods. The method involved solvent extraction, separation was achieved in gradient mode using water with 0.5% formic acid and acetonitrile with 0.5% formic acid and using a reverse phase C18 column. The electrospray ion source operated in negative and positive mode for the detection of glucosinolates and isothiocyanates, respectively, and the multiple reaction monitoring (MRM) was selected as acquisition mode. The method was validated following the ICH guidelines. Replicate experiments demonstrated a good accuracy (bias%<10%) and precision (CV%<10%). Detection limits and quantification limits are in the range of 1-400ng/mL for each analytes. Calibration curves were validated on concentration ranges from 0.05 to 50µg/mL. The method proved to be suitable for glucosinolates and isothiocyanates determination both in biomasses and in complex matrices such as food products enriched with glucosinolates, or nutraceutical bakery products. In addition, the developed method was applied to the simultaneous determination of glucosinolates and isothiocyanates in bakery product enriched with glucosinolates, to evaluate their thermal stability after different industrial processes from cultivation phases to consumer processing. |
| ISSN | 00219673 |
| Volume Number | 1428 |
| e-ISSN | 18733778 |
| Journal | Journal of Chromatography A |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-01-08 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Brassicaceae Chemistry Chromatography, Liquid Food Analysis Methods Functional Food Analysis Glucosinolates Isothiocyanates Spectrometry, Mass, Electrospray Ionization Instrumentation Limit Of Detection Seeds Tandem Mass Spectrometry Journal Article Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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