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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Sanz-Medel, Alfredo Fernández-Menéndez, Sonia Fernández-Colomer, Belén Braz, Bernardo Ferreira Fernández-Sánchez, María L. Freire, Aline Soares de la Flor St Remy, Rafael R. Santelli, Ricardo Erthal Cotallo, Gil Daniel Coto |
| Spatial Coverage | Brazil |
| Description | Country affiliation: Spain Author Affiliation: Fernández-Menéndez S ( Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Oviedo, Spain.); Fernández-Sánchez ML ( Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Oviedo, Spain. Electronic address: marisafs@uniovi.es.); Fernández-Colomer B ( Service of Neonatology, Department of Pediatrics, Hospital Universitario Central de Asturias, Oviedo, Spain.); de la Flor St Remy RR ( Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Oviedo, Spain.); Cotallo GD ( Service of Neonatology, Department of Pediatrics, Hospital Universitario Central de Asturias, Oviedo, Spain.); Freire AS ( Department of Analytical Chemistry, Universidade Federal do Rio de Janeiro, Brazil.); Braz BF ( Department of Analytical Chemistry, Universidade Federal do Rio de Janeiro, Brazil.); Santelli RE ( Department of Analytical Chemistry, Universidade Federal do Rio de Janeiro, Brazil.); Sanz-Medel A ( Department of Physical and Analytical Chemistry, Faculty of Chemistry, University of Oviedo, Oviedo, Spain. Electronic address: asm@uniovi.es.) |
| Abstract | This paper summarises results of zinc content and its speciation in human milk from mothers of preterm and full-term infants at different stages of lactation and from synthetic formula milks. Human milk samples (colostrum, 7th, 14th, and 28th day after delivery) from Spanish and Brazilian mothers of preterm and full-term infants (and also formula milks) were collected. After adequate treatment of the sample, total Zn was determined, while speciation analysis of the Zn was accomplished by size exclusion chromatography coupled online with the ICP-MS. It is observed that total zinc content in human milk decreases continuously during the first month of lactation, both for preterm and full term gestations. All infant formulas analysed for total Zn were within the currently legislated levels. For Zn speciation analysis, there were no differences between preterm and full term human milk samples. Moreover Zn species elute mainly associated with immunoglobulins and citrate in human milk whey. Interestingly the speciation in formula milk whey turned out to be completely different as the observed Zn(2+) was bound almost exclusively to low molecular weight ligands (citrate) and only comparatively very low amounts of the metal appeared to be associated with higher mass biomolecules (e.g. proteins). |
| ISSN | 00219673 |
| Volume Number | 1428 |
| e-ISSN | 18733778 |
| Journal | Journal of Chromatography A |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-01-08 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Chromatography, Gel Food Analysis Methods Infant Formula Chemistry Mass Spectrometry Milk, Human Zinc Analysis Brazil Citrates Humans Infant Molecular Weight Journal Article Research Support, Non-u.s. Gov't Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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