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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Xu, Chengning Cao, Xuejun Dong, Wenying Wan, Junfen |
| Description | Country affiliation: China Author Affiliation: Xu C ( State Key Laboratory of Bioreactor Engineering, Department of Bioengineering, East China University of Science and Technology, Shanghai, 200237, China.); Dong W ( State Key Laboratory of Bioreactor Engineering, Department of Bioengineering, East China University of Science and Technology, Shanghai, 200237, China.); Wan J ( State Key Laboratory of Bioreactor Engineering, Department of Bioengineering, East China University of Science and Technology, Shanghai, 200237, China. Electronic address: wanjunfen@ecust.edu.cn.); Cao X ( State Key Laboratory of Bioreactor Engineering, Department of Bioengineering, East China University of Science and Technology, Shanghai, 200237, China. Electronic address: caoxj@ecust.edu.cn.) |
| Abstract | Aqueous two-phase systems (ATPS) have the potential application in bioseparation and biocatalysis engineering. In this paper, a recyclable ATPS was developed by two thermo-responsive copolymers, P and P . Copolymer P was copolymerized using N-vinylcaprolactam, Butyl methacrylate and Acrylamide as monomers, and P was synthesized by N-isopropylacrylamide. The lower critical solution temperature (LCST) of P and P were 45.0°C and 33.5°C, respectively. The recoveries of both polymers could achieve over 95.0%. The phase behavior and formation mechanism of P /P ATPS was studied. Low-field nuclear magnetic resonance (LF-NMR) was applied in the phase-forming mechanism study in ATPS. In addition, combining the analysis results of surface tension, transmission electron microscopy and dynamic light scattering, the phase-forming of the P /P ATPS was proved. The application was performed by partition of ε-polylysine in the 2% P /2% P (w/w) ATPS. The results demonstrated that ε-polylysine was extracted into the P -rich phase, the maximal partition coefficient (1/K) and extraction recovery of pure ε-polylysine were 6.87 and 96.36%, respectively, and 7.41 partition coefficient and 97.85% extraction recovery for ε-polylysine fermentation broth were obtained in the presence of 50mM (NH ) SO at room temperature. And this method can effectively remove the most impurities from fermentation broth when (NH ) SO exists in the ATPS. It is believed that the thermo-responsive recycling ATPS has a good application prospect in the field of bio-separation. |
| ISSN | 00219673 |
| Volume Number | 1472 |
| e-ISSN | 18733778 |
| Journal | Journal of Chromatography A |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-11-11 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Polylysine Chemistry Isolation & Purification Polymers Chemical Synthesis Recycling Methods Temperature Water Acrylamide Acrylamides Caprolactam Analogs & Derivatives Magnetic Resonance Spectroscopy Methacrylates Solutions Surface Tension Journal Article Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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