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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Jun, Young-Si Hong, Yeon Ki Lee, Eun Zoo Won, Hyo Jin Huh, Yun Suk Park, Tae Jung Lee, Sang Yup Hong, Won Hi |
| Description | Country affiliation: South Korea Author Affiliation: Lee EZ ( Department of Chemical and Biomolecular Engineering (BK21 program), KAIST, 335 Gwahangno, Yuseong-gu, Daejeon 305-701, South Korea.) |
| Abstract | Serum albumin, one of the most abundant serum proteins, blocks the expression of other important biomarkers. The objective of this study is to remove serum albumin effectively by using solid-phase extraction (SPE) in microfluidic devices. Photo-polymerized adsorbent as a stationary phase of SPE was used to remove bovine serum albumin (BSA). The adsorption capacity was examined with the effect of pH and concentration in BSA solution, and adjustment of monomer concentration such as hydrophilic 2-acrylamido-2-methyl-1-propanesulfonic acid and acrylamide in the adsorbent. The effect of hydrophobic butyl methacylate on BSA adsorption was also studied. Selective removal in a bicomponent with BSA and bovine gamma-globulin was performed by adjusting the pH as required. |
| ISSN | 00219673 |
| Issue Number | 1-2 |
| Volume Number | 1187 |
| e-ISSN | 18733778 |
| Journal | Journal of Chromatography A |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2008-04-11 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Serum Albumin, Bovine Isolation & Purification Solid Phase Extraction Methods Acrylamide Radiation Effects Acrylamides Adsorption Alkanesulfonates Hydrogen-ion Concentration Methacrylates Microfluidic Analytical Techniques Instrumentation Microscopy, Electron, Scanning Photochemistry Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Discipline Analytical Chemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Biochemistry |
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