NDLI: Development and validation of ultra-high-performance liquid chromatography-tandem mass spectrometry methods for the simultaneous determination of beauvericin, enniatins (A, A1, B, B1) and cereulide in maize, wheat, pasta and rice.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Madder, Annemieke Sas, Benedikt Decleer, Marlies Rajkovic, Andreja De Saeger, Sarah
Description	Author Affiliation: Decleer M ( Department of Bioanalysis, Laboratory of Food Analysis, Ghent University, Ottergemsesteenweg 460, Ghent, Belgium); Rajkovic A ( Department of Food Safety and Food Quality, Laboratory of Food Microbiology and Food Preservation, Ghent University, Coupure Links 653, Ghent, Belgium.); Sas B ( Department of Food Safety and Food Quality, Food2Know, Ghent University, Coupure Links 653, Ghent, Belgium.); Madder A ( Department of Organic Chemistry, Organic and Biomimetic Chemistry Research Group, Ghent University, Krijgslaan 281, Ghent, Belgium.); De Saeger S ( Department of Bioanalysis, Laboratory of Food Analysis, Ghent University, Ottergemsesteenweg 460, Ghent, Belgium.)
Abstract	Rapid and accurate UPLC-MS/MS methods for the simultaneous determination of beauvericin and the related enniatins (A, A1, B, B1), together with cereulide were successfully developed and validated in cereal and cereal-based food matrices such as wheat, maize, rice and pasta. Although these emerging foodborne toxins are of different microbial origin, the similar structural, toxicological and food safety features provided rationale for their concurrent detection in relevant food matrices. A Waters Acquity UPLC system coupled to a Waters Quattro Premier XE™ Mass Spectrometer operating in ESI+ mode was employed. Sample pretreatment involved a fast and simple liquid extraction of the target toxins without any further clean-up step. For all toxins the sample preparation resulted in acceptable extraction recoveries with values of 85-105% for wheat, 87-106% for maize, 84-106% for rice and 85-105% for pasta. The efficient extraction protocol, together with a fast chromatographic separation of 7min allowed substantial saving costs and time showing its robustness and performance. The validation of the developed method was performed based on Commission Decision 2002/657/EC. The obtained limits of detection ranged from 0.1 to 1.0µgkg and the limits of quantification from 0.3 to 2.9µgkg for the targeted toxins in the selected matrices. The obtained sensitivities allow detection of relevant toxicological concentrations. All relative standard deviations for repeatability (intra-day) and intermediate precision (inter-day) were lower than 20%. Trueness, expressed as the apparent recovery varied from 80 to 107%. The highly sensitive and repeatable validated method was applied to 57 naturally contaminated samples allowing detection of sub-clinical doses of the toxins.
ISSN	00219673
Journal	Journal of Chromatography A
Volume Number	1472
e-ISSN	18733778
Language	English
Publisher	Elsevier
Publisher Date	2016-11-11
Publisher Place	Netherlands
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Medicine Analytical Chemistry Biochemistry

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