NDLI: Construction and validation of metagenomic DNA libraries from landfarm soil microorganisms.

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Genetic variations in the Wnt signaling pathway affect lung function in asthma patients.

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Karyotype studies on populations of two Hypochaeris species (H. catharinensis and H. lutea), Asteraceae, endemics to southern Brazil.

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Construction and validation of metagenomic DNA libraries from landfarm soil microorganisms.

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Construction and validation of metagenomic DNA libraries from landfarm soil microorganisms.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Dias, J. C. T. Rezende, R. P. Cerqueira, A. F. Pessoa, T. B. A. de Souza, S. S. Pirovani, C. P.
Spatial Coverage	Brazil
Description	Country affiliation: Brazil Author Affiliation: Pessoa TB ( Laboratório de Monitoramento Ambiental, Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Salobrinho, Ilhéus, BA, Brasil.)
Abstract	Landfarming biodegradation is a strategy used by the petrochemical industry to reduce pollutants in petroleum-contaminated soil. We constructed 2 metagenomic libraries from landfarming soil in order to determine the pathway used for mineralization of benzene and to examine protein expression of the bacteria in these soils. The DNA of landfarm soil, collected from Ilhéus, BA, Brazil, was extracted and a metagenomic library was constructed with the Copy Control(TM) Fosmid Library Production Kit, which clones 25-45-kb DNA fragments. The clones were selected for their ability to express enzymes capable of cleaving aromatic compounds. These clones were grown in Luria-Bertani broth plus L-arabinose, benzene, and chloramphenicol as induction substances; they were tested for activity in the catechol cleavage pathway, an intermediate step in benzene degradation. Nine clones were positive for ortho-cleavage and one was positive for meta-cleavage. Protein band patterns determined by SDS-polyacrylamide gel electrophoresis differed in bacteria grown on induced versus non-induced media (Luria-Bertani broth). We concluded that the DNA of landfarm soil is an important source of genes involved in mineralization of xenobiotic compounds, which are common in gasoline and oil spills. Metagenomic library allows identification of non-culturable microorganisms that have potential in the bioremediation of contaminated sites.
e-ISSN	16765680
Journal	Genetics and Molecular Research
Issue Number	2
Volume Number	12
Language	English
Publisher	Fundação de Pesquisas Científicas de Ribeirão Preto
Publisher Date	2013-06-28
Publisher Place	Brazil
Access Restriction	Open
Subject Keyword	Bacteria Enzymology Genetics Gene Library Metagenomics Soil Microbiology Metabolism Biodegradation, Environmental Dna, Bacterial Hydrocarbons, Aromatic Petroleum Soil Pollutants Xenobiotics Research Support, Non-u.s. Gov't Validation Studies Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics
Content Type	Text
Resource Type	Article

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