NDLI: Complementary DNA cloning, sequence analysis, and tissue transcription profile of a novel U2AF2 gene from the Chinese Banna mini-pig inbred line.

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Complementary DNA cloning, sequence analysis, and tissue transcription profile of a novel U2AF2 gene from the Chinese Banna mini-pig inbred line.

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Complementary DNA cloning, sequence analysis, and tissue transcription profile of a novel U2AF2 gene from the Chinese Banna mini-pig inbred line.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Cheng, W. M. Wang, S. Y. Zeng, Y. Z. Huo, J. L. Miao, Y. W.
Description	Country affiliation: China Author Affiliation: Wang SY ( Key Laboratory of Banna Mini-Pig Inbred Line of Yunnan Province, Kunming, Yunnan, China.)
Abstract	U2 small nuclear RNA auxiliary factor 2 (U2AF2) is an important gene for pre-messenger RNA splicing in higher eukaryotes. In this study, the Banna mini-pig inbred line (BMI) U2AF2 coding sequence (CDS) was cloned, sequenced, and characterized. The U2AF2 complete CDS was amplified using the reverse transcription-polymerase chain reaction (RT-PCR) technique based on the conserved sequence information of cattle and known highly homologous swine expressed sequence tags. This novel gene was deposited into the National Center for Biotechnology Information database (Accession No. JQ839267). Sequence analysis revealed that the BMI U2AF2 coding sequence consisted of 1416 bp and encoded 471 amino acids with a molecular weight of 53.12 kDa. The protein sequence has high sequence homology with U2AF65 of 6 species - Homo sapiens (100%), Equus caballus (100%), Canis lupus (100%), Macaca mulatta (99.8%), Bos taurus (74.4%), and Mus musculus (74.4%). The phylogenetic tree analysis revealed that BMI U2AF65 has a closer genetic relationship with B. taurus U2AF65 than with U2AF65 of E. caballus, C. lupus, M. mulatta, H. sapiens, and M. musculus. RT-PCR analysis showed that BMI U2AF2 was most highly expressed in the brain; moderately expressed in the spleen, lung, muscle, and skin; and weakly expressed in the liver, kidney, and ovary. Its expression was nearly silent in the spinal cord, nerve fiber, heart, stomach, pancreas, and intestine. Three microRNA target sites were predicted in the CDS of BMI U2AF2 messenger RNA. Our results establish a foundation for further insight into this swine gene.
e-ISSN	16765680
Journal	Genetics and Molecular Research
Issue Number	2
Volume Number	12
Language	English
Publisher	Fundação de Pesquisas Científicas de Ribeirão Preto
Publisher Date	2013-04-02
Publisher Place	Brazil
Access Restriction	Open
Subject Keyword	Cloning, Molecular Dna, Complementary Gene Expression Profiling Gene Expression Regulation Nuclear Proteins Chemistry Genetics Ribonucleoproteins Amino Acid Sequence Animals Evolution, Molecular Micrornas Metabolism Molecular Sequence Data Organ Specificity Phylogeny Protein Interaction Domains And Motifs Protein Structure, Secondary Sequence Alignment Sequence Analysis, Dna Swine Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics
Content Type	Text
Resource Type	Article

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