| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Wu, J. P. Jia, J. L. Ha, Z. J. Li, W. W. Zhang, L. P. |
| Description |
Country affiliation: China Author Affiliation: Jia JL ( Key Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.); Zhang LP ( Key Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China zhangliping@gsau.edu.cn.); Wu JP ( Key Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.); Ha ZJ ( Key Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.); Li WW ( Key Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, Gansu Agricultural University, Lanzhou, Gansu, China.) |
| Abstract |
The growth hormone gene plays an important role in the physiological function of an organism. The current study aimed to investigate the correlation between polymorphisms in the 5' regulatory region, exon 4, and 3' untranslated region (UTR) of the sheep GH gene and sheep growth traits. The DNA from 510 adult sheep was analyzed by DNA sequencing and polymerase chain reaction single-strand conformation polymorphism. Two alleles (A and B) and 3 genotypes (AA, AB, and BB), 2 alleles (A and B) and 3 genotypes (AA, AB, and BB), and 3 alleles (A, B, and C) and 4 genotypes (AA, AB, BB, and AC) were found within the 5' regulatory region, exon 4, and 3' UTR, respectively. In Tibetan sheep, the association analysis indicated that there were statistically significant differences in the scores of weight, length, and heart girth within the 5' regulatory region; weight, length, wither height, and heart girth within exon 4; and weight, length, wither height, and heart girth within the 3' UTR among the different genotypes. For exon 4, Poll Dorset sheep individuals with genotype AA showed a lower score than those of genotypes BB and AB (P<0.05). With regard to the 3' UTR, Poll Dorset sheep with genotype AC showed higher scores than those of genotypes AA and AB (P<0.05). |
| e-ISSN |
16765680 |
| Journal |
Genetics and Molecular Research |
| Issue Number |
3 |
| Volume Number |
13 |
| Language |
English |
| Publisher |
Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date |
2014-09-05 |
| Publisher Place |
Brazil |
| Access Restriction |
Open |
| Subject Keyword |
Growth Hormone Genetics Polymorphism, Genetic Quantitative Trait, Heritable Growth & Development 3' Untranslated Regions Alleles Animals Exons Gene Frequency Genetic Association Studies Genotype Molecular Sequence Data Polymorphism, Single Nucleotide Sequence Analysis, Dna Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type |
Text |
| Resource Type |
Article |
