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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Jiang, W-P Zhao, J-L Li, Q. Gu, Z-M Li, F. Jia, Y-Y Liu, S-L |
| Description | Country affiliation: China Author Affiliation: Liu SL ( Zhejiang Institute of Freshwater Fisheries, HuZhou, China.); Gu ZM ( Zhejiang Institute of Freshwater Fisheries, HuZhou, China guzhimin2006@163.com.); Jia YY ( Zhejiang Institute of Freshwater Fisheries, HuZhou, China.); Zhao JL ( Key Laboratory of Freshwater Fisheries Germplasm Resource, Shangai Ocean University, Ministry of Agriculture, Shanghai, China.); Jiang WP ( Zhejiang Institute of Freshwater Fisheries, HuZhou, China.); Li Q ( Zhejiang Institute of Freshwater Fisheries, HuZhou, China.); Li F ( Zhejiang Institute of Freshwater Fisheries, HuZhou, China.) |
| Abstract | The topmouth culter (Culter alburnus) is an economically important freshwater fish, which is widely distributed throughout large rivers, reservoirs, and lake areas of China. We report here the isolation and characterization of 32 new polymorphic microsatellite loci isolated from genomic DNA in this species enriched by (CA)12 and (GA)12 probes. The variability of these microsatellites was tested on 30 individuals cultured. The average allele number was 6.6 per locus, ranging from 3 to 12. The observed heterozygosity was from 0.4667 to 0.9000, and the expected heterozygosity was from 0.6163 to 0.9085. After using Bonferroni's correction for multiple tests, there was no evidence of linkage disequilibrium between pairs of loci, but deviations from Hardy-Weinberg equilibrium were found in 3 loci. These microsatellites can be used to study QTL of economic importance, population genetic diversity and the construction of genetic maps for C. alburnus in the future. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 3 |
| Volume Number | 13 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2014-09-12 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Genetics Genetic Loci Microsatellite Repeats Alleles Animals Classification Genetic Heterogeneity Molecular Sequence Data Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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