| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Jiang, Z. Y. Yang, Y. F. Yang, Z. J. Sun, Q. X. Wang, Q. H. Wei, S. H. Hu, Z. S. Peng, Z. S. |
| Description |
Country affiliation: China Author Affiliation: Wang QH ( The Ministry of Education Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Science, China West Normal University, Nanchong City, Sichuan, China.); Yang ZJ ( The Ministry of Education Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Science, China West Normal University, Nanchong City, Sichuan, China.); Wei SH ( The Ministry of Education Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Science, China West Normal University, Nanchong City, Sichuan, China.); Jiang ZY ( The Ministry of Education Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Science, China West Normal University, Nanchong City, Sichuan, China.); Yang YF ( The Ministry of Education Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Science, China West Normal University, Nanchong City, Sichuan, China.); Hu ZS ( The Ministry of Education Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Science, China West Normal University, Nanchong City, Sichuan, China.); Sun QX ( The Ministry of Education Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Science, China West Normal University, Nanchong City, Sichuan, China.); Peng ZS ( The Ministry of Education Key Laboratory of Southwest China Wildlife Resources Conservation, College of Life Science, China West Normal University, Nanchong City, Sichuan, China.) |
| Abstract |
Wheat WAG-1 is a C-class MADS-box gene, which is orthologous to AGAMOUS in Arabidopsis. In this study, we report the cloning, characterization, and expression patterns of WAG-1 in the pistillody mutant HTS-1 and its sib-line CSTP. The cDNA of WAG-1 was found to be 765 bp in length, which was equal to the length of its open reading frame, encoding 254 amino acids. The location of WAG-1 revealed that it has three homologous genes from the short arm of chromosome 1A, 1B, and 1D. Their genomic sequences were determined to be 5864, 6454, and 6447 bp long, respectively, and possessed seven exons and six introns. Young spikes from HTS-1 contained higher levels of WAG-1 transcript than did those from CSTP, and the transcript levels in the young spikes (7-10 mm in length) of HTS-1 increased 3.3-fold relative to those of the CSTP line. The transcript level in the pistil and pistil-like stamens of HTS-1 was over 2-fold higher than that in the stamens of CSTP, and expression in the pistil-like stamens of HTS-1 was slightly higher than that in its pistils. These data provide a basis for future research into the function of WAG-1, and offer further insight into the molecular mechanism of the pistillody mutation in common wheat. |
| e-ISSN |
16765680 |
| Journal |
Genetics and Molecular Research |
| Issue Number |
4 |
| Volume Number |
14 |
| Language |
English |
| Publisher |
Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date |
2015-10-16 |
| Publisher Place |
Brazil |
| Access Restriction |
Open |
| Subject Keyword |
Metabolism Mads Domain Proteins Plant Proteins Triticum Cloning, Molecular Gene Expression Regulation, Plant Genetics Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type |
Text |
| Resource Type |
Article |
