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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Domnanich, Patrick Preininger, Claudia Peña, Dacimoneida Brito |
| Description | Country affiliation: Austria Author Affiliation: Domnanich P ( AIT Austrian Institute of Technology GmbH, Health & Environment/Bioresources, 2444 Seibersdorf, Austria.) |
| Abstract | Protein microarrays for disease diagnostics are required to accurately quantify analytes in the low pg/mL range. This task is hampered by weak signal strengths and too low detector sensitivity. Herein we present reflective gold chips coated with polyelectrolyte multilayers (PEMs) for signal enhancement in immunoassays for melanoma-relevant biomarkers. Among tested (semi)natural polysaccharides (xanthan, chitosan, carboxymethylcellulose, hyaluronic acid) PEMs composed of xanthan and chitosan performed best in terms of detection of low analyte concentrations (ED10), spot morphology, fluorescence background and variability (<10%). Fluorescence signals on gold slides with a 75 nm coating of seven crosslinked polyelectrolyte double layers were up to 50 times higher than on bare glass slides. In comparison to commercial substrates the signal to noise ratio is enhanced by up to factor 11. Furthermore sandwich assays for interleukins 6, 8, 10, tumour necrosis factor alpha (TNF ), vascular endothelial growth factor A (VEGF-A) and S100B show working ranges which cover significantly lower concentrations (up to 38-fold). Not limited to above assays the presented substrates, which combine a biocompatible interface with metal-based signal amplification, are a valuable tool in a variety of biosensor applications. |
| ISSN | 09565663 |
| Issue Number | 5 |
| Volume Number | 26 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-01-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Tumor Markers, Biological Analysis Chitosan Chemistry Immunoassay Instrumentation Melanoma Metabolism Neoplasm Proteins Polysaccharides, Bacterial Protein Array Analysis Biosensing Techniques Cell Line, Tumor Equipment Design Equipment Failure Analysis Gold Humans Diagnosis Spectrometry, Fluorescence Journal Article Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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