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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | McNaughton, Brandon H. Newton, Duane W. Kinnunen, Paivo Sinn, Irene Burns, Mark A. Kopelman, Raoul |
| Description | Country affiliation: United States Author Affiliation: Kinnunen P ( The University of Michigan, Department of Chemistry, 930 N. University, Ann Arbor, MI 48109-1055, USA. pkkinn@umich.edu) |
| Abstract | Continuous growth of individual bacteria has been previously studied by direct observation using optical imaging. However, optical microscopy studies are inherently diffraction limited and limited in the number of individual cells that can be continuously monitored. Here we report on the use of the asynchronous magnetic bead rotation (AMBR) sensor, which is not diffraction limited. The AMBR sensor allows for the measurement of nanoscale growth dynamics of individual bacterial cells, over multiple generations. This torque-based magnetic bead sensor monitors variations in drag caused by the attachment and growth of a single bacterial cell. In this manner, we observed the growth and division of individual Escherichia coli, with 80-nm sensitivity to the cell length. Over the life cycle of a cell, we observed up to a 300% increase in the rotational period of the biosensor due to increased cell volume. In addition, we observed single bacterial cell growth response to antibiotics. This work demonstrates the non-microscopy limited AMBR biosensor for monitoring individual cell growth dynamics, including cell elongation, generation time, lag time, and division, as well as their sensitivity to antibiotics. |
| ISSN | 09565663 |
| e-ISSN | 18734235 |
| DOI | 10.1016/j.bios.2010.10.010 |
| Journal | Biosensors and Bioelectronics |
| Issue Number | 5 |
| Volume Number | 26 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-01-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | Open |
| Subject Keyword | Anti-bacterial Agents Pharmacology Escherichia Coli Drug Effects Growth & Development Immunomagnetic Separation Instrumentation Transducers Cell Survival Equipment Design Equipment Failure Analysis Rotation Research Support, N.i.h., Extramural Research Support, U.s. Gov't, Non-p.h.s. Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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