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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Studer, A. Demarche, S. Langenegger, D. Tiefenauer, L. |
| Description | Country affiliation: Switzerland Author Affiliation: Studer A ( Paul Scherrer Institut, Department of Biology and Chemistry, Biomolecular Research Laboratory, CH-5232 Villigen PSI, Switzerland. a.studer@imperial.ac.uk) |
| Abstract | Functional assays for membrane proteins become increasingly important in biosciences. We demonstrate the integration of reconstituted bacterial voltage-gated sodium channels (NaChBac) into preformed free-standing lipid bilayers by using the nystatin-ergosterol method to promote proteoliposome fusion. Vesicle delivery and subsequent NaChBac activity were monitored, the orientation of the transferred ion channels was assessed measuring at both, positive and negative holding potentials and the channel specificity was demonstrated by adding the blocker nimodipine. A conductance of 120 pS per channel and an opening time in the range of seconds have been observed. Interestingly, we found that fusion of proteoliposomes into preformed free-standing bilayers is limited, if hydrophobically silanized silicon nitride membranes are used as the supporting material. In this case the diameter of the liposome had to be at least 20 times smaller compared to that of the pore to render fusion possible. |
| ISSN | 09565663 |
| Issue Number | 5 |
| Volume Number | 26 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-01-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Bacterial Proteins Chemistry Biomimetic Materials Biosensing Techniques Instrumentation Conductometry Ion Channel Gating Lipid Bilayers Membrane Potentials Sodium Channels Equipment Design Equipment Failure Analysis Systems Integration Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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