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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Yasukawa, Tomoyuki Ramón-Azcón, Javier Mizutani, Fumio |
| Description | Country affiliation: Japan Author Affiliation: Ramón-Azcón J ( Graduate School of Material Science, University of Hyogo, 3-2-1 Kouto, Kamigori, Ako, Hyogo 678-1297, Japan.) |
| Abstract | In this study, a rapid immunosensing system has been developed for simultaneous analysis of two tumor markers, alpha-fetoprotein (AFP) and prostate-specific antigen (PSA). The strategy for rapid multisensing is based on rapid immunoreactions occurring on the surface of microparticles and the spatial separation of different particles that exhibit distinct dielectrophoretic (DEP) properties. Recognition events for immunoreactions have been performed on the surfaces of two different microparticles conjugated with two different antibodies: polystyrene (PS) microparticles with an anti-AFP antibody and gold-coated (50 nm) PS microparticles with an anti-PSA antibody. The DEP devices consisted of an upper indium tin oxide (ITO) glass and a lower ITO electrode with a castellated structure. Sandwich structured immunocomplexes of AFP and PSA were created on the microparticles and then labeled with fluorescent molecules via a secondary antibody. After introducing the particles into the DEP devices, an alternating current (AC) voltage (20 V peak-to-peak voltage and 30 kHz) was applied between the upper ITO and lower electrodes to manipulate the particles with negative dielectrophoresis (n-DEP).The uncoated PS particles and the gold-coated PS particles rapidly moved and separated to form wave-like line and triangular aggregates, respectively. The measurements of the fluorescence signals from the uncoated and gold-coated PS particles directed to different regions of the DEP device permit the determination of the concentrations of AFP and PSA simultaneously. No cross-reactivity was observed for either of the immunorecognition events. Limits of detection achieved for the AFP and PSA assays were 0.18 and 1.1 ng mL(-1), respectively, which satisfy medical requirements for both antigens in human serum. The total assay time required for the simultaneous detection of the two different analytes in this study (25 min) was shortened compared to the conventional enzyme-linked immunosorbent assay. |
| ISSN | 09565663 |
| Issue Number | 1 |
| Volume Number | 28 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-10-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Tumor Markers, Biological Blood Biosensing Techniques Instrumentation Electrophoresis Methods Immunoassay Prostate-specific Antigen Alpha-fetoproteins Analysis Calibration Electrodes Gold Chemistry Humans Polystyrenes Spectrometry, Fluorescence Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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