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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Luo, Yueping Feng, Bo Dai, Youzhi Huang, Shirong Ge, Fei Jia, Dayong |
| Description | Country affiliation: China Author Affiliation: Feng B ( College of Chemical Engineering, Xiangtan University, Xiangtan 411105, Hunan Province, China. fengbo@xtu.edu.cn) |
| Abstract | The amount of active capture antibodies immobilized per unit square is crucial to developing effective antibody chips, biosensors, immunoassays and other molecular recognition technologies. In this study, we present a novel yet simple method for oriented antibody immobilization at high density based on the formation of an orderly, organized aggregation of immunoglobulin G (IgG) and staphylococcal protein A (SPA). Following the chelation of His-tag with Ni(2+), antibodies were immobilized on a solid surface in a three-dimensional (3D) manner and exposed the analyte receptor sites well, which resulted in a substantial enhancement of the analytical signal with more than 64-fold increase in detection sensitivity. Pull-down assays confirmed that IgG antibody can only bind to Ni(2+) matrix indirectly via a SPA linkage, where the His-tag is responsible for binding Ni(2+) and homologous domains are responsible for binding IgG Fc. The immobilization approach proposed here may be an attractive strategy for the construction of high performance antibody arrays and biosensors as long as the antibody probe is of mammalian IgG. |
| ISSN | 09565663 |
| Issue Number | 1 |
| Volume Number | 28 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-10-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Antibodies, Immobilized Chemistry Enzyme-linked Immunosorbent Assay Methods Hepatitis B e Antigens Analysis Immunoglobulin G Staphylococcal Protein A Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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