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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Torimitsu, Keiichi Tamba, Yukihiro Shinozaki, Youichi McAllister, Arianna Tanaka, Aya Sumitomo, Koji Kashimura, Yoshiaki |
| Description | Country affiliation: Japan Author Affiliation: Sumitomo K ( NTT Basic Research Laboratories, NTT Corporation, 3-1 Morinosato Wakamiya, Atsugi, Kanagawa 243-0198, Japan. sumitomo.koji@lab.ntt.co.jp) |
| Abstract | For the functional analysis of ion channel activity, an artificial lipid bilayer suspended over microwells was formed that ruptured giant unilamellar vesicles on a Si substrate. Ca(2+) ion indicators (fluo-4) were confined in the microwells by sealing the microwells with a lipid bilayer. An overhang formed at the microwells prevented the lipid membrane from falling into them and allowed the stable confinement of the fluorescent probes. The transport of Ca(2+) ions through the channels formed by -hemolysin inserted in a lipid membrane was analyzed by employing the fluorescence intensity change of fluo-4 in the microwells. The microwell volume was very small (1-100 fl), so a highly sensitive monitor could be realized. The detection limit is several tens of ions/s/µm(2), and this is much smaller than the ion current in a standard electrophysiological measurement. Smaller microwells will make it possible to mimic a local ion concentration change in the cells, although the signal to noise ratio must be further improved for the functional analysis of a single channel. We demonstrated that a microwell array with confined fluorescent probes sealed by a lipid bilayer could constitute a basic component of a highly sensitive biosensor array that works with functional membrane proteins. This array will allow us to realize high throughput and parallel testing devices. |
| ISSN | 09565663 |
| Issue Number | 1 |
| Volume Number | 31 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-01-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Bacterial Toxins Chemistry Calcium Fluorescent Dyes Hemolysin Proteins Ion Channel Gating Lipid Bilayers Microarray Analysis Instrumentation Microfluidic Analytical Techniques Equipment Design Equipment Failure Analysis Analysis Ion Transport Reproducibility Of Results Sensitivity And Specificity Silicon Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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