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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ng, Hui Hwee Stulz, Eugen Park, Mi Kyoung Kongsuphol, Patthara Wong, Chee Chung Pursey, Joanna P. Arya, Sunil K. |
| Description | Author Affiliation: Kongsuphol P ( Institute of Microelectronics, A(â )STAR (Agency for Science Technology and Research), 11 Science Park Road, Singapore Science Park II, 117685, Singapore. Electronic address: kongsupholp@ime.a-star.edu.sg.); Ng HH ( Institute of Microelectronics, A(â )STAR (Agency for Science Technology and Research), 11 Science Park Road, Singapore Science Park II, 117685, Singapore.); Pursey JP ( Institute of Microelectronics, A(â )STAR (Agency for Science Technology and Research), 11 Science Park Road, Singapore Science Park II, 117685, Singapore); Arya SK ( Institute of Microelectronics, A(â )STAR (Agency for Science Technology and Research), 11 Science Park Road, Singapore Science Park II, 117685, Singapore.); Wong CC ( Institute of Microelectronics, A(â )STAR (Agency for Science Technology and Research), 11 Science Park Road, Singapore Science Park II, 117685, Singapore.); Stulz E ( School of Chemistry and Institute for Life Sciences, University of Southampton, Highfield, Southampton SO17 1BJ, UK.); Park MK ( Institute of Microelectronics, A(â )STAR (Agency for Science Technology and Research), 11 Science Park Road, Singapore Science Park II, 117685, Singapore.) |
| Abstract | Serum background is a critical issue for biosensor development as it interferes with the detection of target molecules and may give rise to false positive signal. We present here highly sensitive and selective TNF- biosensor which is able to detect TNF- from non-diluted human serum using magnetic bead coupled antibody and electrochemical impedance spectroscopy (EIS) techniques. The process is designed to detect TNF- from human serum in three stages; (1) abundant protein backgrounds are depleted from the serum using magnetic bead coupled albumin and IgG antibodies, (2) after background depletion TNF- is captured using magnetic bead coupled TNF- antibody, and (3) the captured TNF- is eluted from the magnetic beads and measured using EIS technique in which comb structured gold microelectrodes array (CSGM) is utilized to enhance the detection sensitivity. The system is able to achieve the limit of detection (LOD) at 1 pg/ml (57 fM) and a linear relationship between increasing TNF- concentrations and charge-transfer resistance in a dynamic range of 1-1000 pg/ml. |
| ISSN | 09565663 |
| Volume Number | 61 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-11-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Methods Tumor Necrosis Factor-alpha Blood Antibodies, Immobilized Chemistry Dielectric Spectroscopy Electrodes Humans Immunomagnetic Separation Limit Of Detection Evaluation Studies Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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