| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Jung, Jae Hwan Cho, Minkyung Chung, Soyi Jeon, Jun Ho Seo, Tae Seok Rhie, Gi-eun |
| Description |
Author Affiliation: Cho M ( Department of Chemical and Biomolecular Engineering (BK21 PLUS Program), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. Electronic address: mkcho25@kaist.ac.kr.); Chung S ( Department of Chemical and Biomolecular Engineering (BK21 PLUS Program), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. Electronic address: csy717@kaist.ac.kr.); Jung JH ( Department of Chemical and Biomolecular Engineering (BK21 PLUS Program), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. Electronic address: jjaehwan@kaist.ac.kr.); Rhie GE ( Division of High-Risk Pathogen Research, Center for Infectious Diseases, National Institute of Health, 187 Osongsaengmyeong 2-ro, Cheongwon-gun, Chungbuk 363-951, Republic of Korea. Electronic address: gerhie@nih.go.kr.); Jeon JH ( Division of High-Risk Pathogen Research, Center for Infectious Diseases, National Institute of Health, 187 Osongsaengmyeong 2-ro, Cheongwon-gun, Chungbuk 363-951, Republic of Korea. Electronic address: jhjeon78@korea.kr.); Seo TS ( Department of Chemical and Biomolecular Engineering (BK21 PLUS Program), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 305-701, Republic of Korea. Electronic address: seots@kaist.ac.kr.) |
| Abstract |
Early diagnosis of biological agents is of paramount importance to prevent the casualties and fatal disease in human during bioterrorism or biological warfare. In this study, we reported an efficient and sensitive multiplex biological agent detection method based on the DNA biobarcode assay and the micro-capillary electrophoresis (µCE) technology. Monoplex as well as multiplex pathogen identification was performed using five targets including Bacillus anthracis, Francisella tularensis, Yersinia pestis, Vaccinia virus and Botulinum toxin A. Through the DNA biobarcode assay process, the magnetic microparticle-pathogen-polystyrene microbead complexes were formed, and the FAM labeled single stranded barcode DNA could be released from the complexes upon denaturation. Different lengths of a barcode DNA were designed to designate each pathogen, so that the specific peak elution time in the capillary electrophoresis on a chip allows us to distinguish the target with high accuracy within 3 min. We improved the assignment accuracy of the peak in the electropherogram by adding two bracket ladders. Owing to the abundant amount of barcode DNAs, the presence of B. anthracis, F. tularensis, Y. pestis, Vaccinia virus was confirmed with a limit of detection of 50CFU/mL, while Botulinum toxin A was analyzed even at a concentration of 12.5 ag/mL. Multiple pathogen detection was also successfully conducted in a phosphate buffered saline (PBS) as well as a serum medium with background of other pathogens. Thus, our analytical platform based on the biobarcode assay and on-chip CE analysis provides rapid, sensitive, multiplex, and accurate biological agent identification. |
| ISSN |
09565663 |
| Volume Number |
61 |
| e-ISSN |
18734235 |
| Journal |
Biosensors and Bioelectronics |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2014-11-15 |
| Publisher Place |
Great Britain (UK) |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Biosensing Techniques Instrumentation Dna, Bacterial Isolation & Purification Dna, Viral Electrophoresis, Capillary Bacillus Anthracis Genetics Bacterial Infections Diagnosis Microbiology Bioterrorism Botulinum Toxins, Type A Equipment Design Francisella Tularensis Humans Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction Vaccinia Vaccinia Virus Yersinia Pestis Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
