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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Yoon, Do Young Schrand, Amanda M. Baker, Peter A. Goltz, Mark N. Kim, Dong-Shik |
| Description | Country affiliation: United States Author Affiliation: Baker PA ( Department of Systems Engineering and Management, Air Force Institute of Technology, WPAFB, OH 45433, USA.); Goltz MN ( Department of Systems Engineering and Management, Air Force Institute of Technology, WPAFB, OH 45433, USA. Electronic address: mark.goltz@afit.edu.); Schrand AM ( Energetic Materials Branch, Munitions Directorate, Air Force Research Laboratory, Eglin AFB, FL 32542, USA.); Yoon DY ( Department of Chemical Engineering, Kwangwoon University, South Korea.); Kim DS ( Department of Chemical and Environmental Engineering, University of Toledo, Toledo, OH 43606, USA.) |
| Abstract | Peptide nanotubes (PNTs) encapsulating horseradish peroxidase and surface coated with acetylcholinesterase (AChE) were attached to gold screen printed electrodes to construct a novel gas phase organophosphate (OP) biosensor. When the sensor with the AChE enzyme is put in contact with acetylthiocholine (ATCh), the ATCh is hydrolyzed to produce thiocholine, which is then oxidized by horseradish peroxidase (HRP). Direct electron transfer between HRP and electrode is achieved through PNTs. The signal produced by the electron transfer is measured with cyclic voltammetry (CV). The presence of an OP compound inhibits this signal by binding with the AChE enzyme. In this study, gas phase malathion was used as a model OP due to the fact that it displays the identical binding mechanism with acetylcholinesterase (AChE) as its more potent counterparts such as sarin and VX, but has low toxicity, making it more practical and safer to handle. The CV signal was proportionally inhibited by malathion vapor concentrations as low as 12 ppbv. Depending on the method used in their preparation, the electrodes maintained their activity for up to 45 days. This research demonstrates the potential of applying nano-modified biosensors for the detection of low levels of OP vapor, an important development in countering weaponized organophosphate nerve agents and detecting commercially-used OP pesticides. |
| ISSN | 09565663 |
| Volume Number | 61 |
| e-ISSN | 18734235 |
| Journal | Biosensors and Bioelectronics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-11-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biosensing Techniques Instrumentation Gases Analysis Gold Chemistry Nanotubes, Peptide Organophosphates Acetylcholinesterase Animals Electrodes Electrophorus Enzymes, Immobilized Equipment Design Horseradish Peroxidase Insecticides Limit Of Detection Malathion Volatilization Evaluation Studies Journal Article Research Support, U.s. Gov't, Non-p.h.s. Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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