NDLI: A fluorescent biosensor based on carbon dots-labeled oligodeoxyribonucleotide and graphene oxide for mercury (II) detection.

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A fluorescent biosensor based on carbon dots-labeled oligodeoxyribonucleotide and graphene oxide for mercury (II) detection.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wu, Jing Zhu, Lei Hou, Yu Cui, Xin Yang, Mei Wang, Peiyao Wang, Zhenni
Description	Country affiliation: China Author Affiliation: Cui X ( College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, Liaoning, China.); Zhu L ( College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, Liaoning, China.); Wu J ( College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, Liaoning, China.); Hou Y ( College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, Liaoning, China.); Wang P ( College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, Liaoning, China.); Wang Z ( College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, Liaoning, China.); Yang M ( College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian 116029, Liaoning, China.)
Abstract	As the newest two members of the carbon materials family, carbon dots (CDs) and graphene oxide (GO) possess many excellent optical properties resulting in a wide range of applications. In this work, we successfully synthesized CDs with a high-quantum-yield, and labeled them on oligodeoxyribonucleotide (ODN). The fluorescence of resultant CDs-labeled oligodeoxyribonucleotide (ODN-CDs) was quenched by GO via fluorescence resonance energy transfer. In the presence of Hg(2+), the fluorescence was recovered by the release of ODN-CDs from GO due to the formation of T-Hg(2+)-T duplex. In the light of this theory, we designed a simple, highly sensitive and selective fluorometric Hg(2+) sensor based on CDs-labeled oligodeoxyribonucleotide and GO without complicated, costly and time-consuming operations. Under the optimal conditions, a linear relationship was obtained between relative fluorescence intensity and the concentration of Hg(2+) in the range of 5-200 nM (R(2)=0.974). The present GO-based sensor system is highly selective toward Hg(2+) over a wide range of metal ions and has a detection limit of 2.6 nM. This method is reliable, and has been successfully applied for the detection of Hg(2+) in practical samples.
ISSN	09565663
Volume Number	63
e-ISSN	18734235
Journal	Biosensors and Bioelectronics
Language	English
Publisher	Elsevier
Publisher Date	2015-01-15
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Biosensing Techniques Instrumentation Fluorescence Resonance Energy Transfer Graphite Chemistry Mercury Analysis Oligodeoxyribonucleotides Quantum Dots Carbon Equipment Design Equipment Failure Analysis Oxides Staining And Labeling Methods Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry

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