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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Kim, Hye Jin Park, Jung Ho Kim, Jinsik Yoo, Yong Kyoung Lee, Jeong Hoon Hwang, Kyo Seon |
| Description | Author Affiliation: Kim HJ ( Center for Biomicrosystems, Korea Institute of Science and Technology (KIST), Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea); Kim J ( Center for Biomicrosystems, Korea Institute of Science and Technology (KIST), Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea.); Yoo YK ( Center for Biomicrosystems, Korea Institute of Science and Technology (KIST), Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea.); Lee JH ( Department of Electrical Engineering, Kwangwoon University, 20 Gwangun-ro, Nowon-gu, Seoul 01897, Republic of Korea.); Park JH ( Department of Electrical Engineering, Korea University,145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea.); Hwang KS ( Center for Biomicrosystems, Korea Institute of Science and Technology (KIST), Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul 02792, Republic of Korea. Electronic address: kshwang@kist.re.kr.) |
| Abstract | Effective control of nano-scale biomolecules can enhance the sensitivity and limit of detection of an interdigitated microelectrode (IME) sensor. Manipulation of the biomolecules by dielectrophoresis (DEP), especially the negative DEP (nDEP) force, so that they are trapped between electrodes (sensing regions) was predicted to increase the binding efficiency of the antibody and target molecules, leading to a more effective reaction. To prove this concept, amyloid beta 42 (Aß42) and prostate specific antigen (PSA) protein were respectively trapped between the sensing region owing to the nDEP force under 5V and 0.05V, which was verified with COMSOL simulation. Using the simulation value, the resistance change (ΔR/Rb) of the IME sensor from the specific antibody-antigen reaction of the two biomolecules and the change in fluorescence intensity were compared in the reference (pDEP) and nDEP conditions. The ΔR/Rb value improved by about 2-fold and 1.66-fold with nDEP compared to the reference condition with various protein concentrations, and these increases were confirmed with fluorescence imaging. Overall, nDEP enhanced the detection sensitivity for Aß42 and PSA by 128% and 258%, respectively, and the limit of detection improved by up to 2-orders of magnitude. These results prove that DEP can improve the biosensor's performance. |
| ISSN | 09565663 |
| Journal | Biosensors and Bioelectronics |
| Volume Number | 85 |
| e-ISSN | 18734235 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-11-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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