NDLI: Polyaniline-graphene based -amylase biosensor with a linear dynamic range in excess of 6 orders of magnitude.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lloyd, Catherine Conlan, R. Steven Yao, Seydou Whitaker, Iain S. Teixeira, Sofia Rodrigues Francis, Lewis Azzopardi, Ernest
Description	Author Affiliation: Teixeira SR ( College of Engineering, Swansea University, Bay Campus, Swansea SA1 8QQ, UK); Lloyd C ( College of Engineering, Swansea University, Bay Campus, Swansea SA1 8QQ, UK); Yao S ( Swansea University Medical School, Singleton Park, Swansea SA2 8PP, UK); Andrea Salvatore Gazze ( Swansea University Medical School, Singleton Park, Swansea SA2 8PP, UK); Whitaker IS ( Swansea University Medical School, Singleton Park, Swansea SA2 8PP, UK); Francis L ( Swansea University Medical School, Singleton Park, Swansea SA2 8PP, UK); Conlan RS ( Swansea University Medical School, Singleton Park, Swansea SA2 8PP, UK); Azzopardi E ( Swansea University Medical School, Singleton Park, Swansea SA2 8PP, UK)
Abstract	-amylase is an established marker for diagnosis of pancreatic and salivary disease, and recent research has seen a substantial expansion of its use in therapeutic and diagnostic applications for infection, cancer and wound healing. The lack of bedside monitoring devices for -amylase detection has hitherto restricted the clinical progress of such applications. We have developed a highly sensitive -amylase immunosensor platform, produced via in situ electropolymerization of aniline onto a screen-printed graphene support (SPE). Covalently binding an -amylase specific antibody to a polyaniline (PANI) layer and controlling device assembly using electrochemical impedance spectroscopy (EIS), we have achieved a highly linear response against -amylase concentration. Each stage of the assembly was characterized using a suite of high-resolution topographical, chemical and mechanical techniques. Quantitative, highly sensitive detection was demonstrated using an artificially spiked human blood plasma samples. The device has a remarkably wide limit of quantification (0.025-1000IU/L) compared to -amylase assays in current clinical use. With potential for simple scale up to volume manufacturing though standard semiconductor production techniques and subsequently clinical application, this biosensor will enable clinical benefit through early disease detection, and better informed administration of correct therapeutic dose of drugs used to treat -amylase related diseases.
ISSN	09565663
Journal	Biosensors and Bioelectronics
Volume Number	85
e-ISSN	18734235
Language	English
Publisher	Elsevier
Publisher Date	2016-11-15
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biotechnology
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry

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