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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Hageneder, Simone Brynda, Eduard Hofner, Manuela Dostálek, Jakub Rodriguez-Emmenegger, Cesar Surman, Frantisek Pop-Georgievski, Ognen Riedel, Tomás Noehammer, Christa |
| Description | Author Affiliation: Riedel T ( Institute of Macromolecular Chemistry AS CR v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic. Electronic address: riedel@imc.cas.cz.); Surman F ( Institute of Macromolecular Chemistry AS CR v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.); Hageneder S ( Biosensor Technologies, AIT-Austrian Institute of Technology GmbH, Muthgasse 11, 1190 Vienna, Austria.); Pop-Georgievski O ( Institute of Macromolecular Chemistry AS CR v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.); Noehammer C ( Molecular Diagnostics, Health and Environment Dpt., AIT-Austrian Institute of Technology GmbH, Muthgasse 11, 1190 Vienna, Austria.); Hofner M ( Molecular Diagnostics, Health and Environment Dpt., AIT-Austrian Institute of Technology GmbH, Muthgasse 11, 1190 Vienna, Austria.); Brynda E ( Institute of Macromolecular Chemistry AS CR v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic.); Rodriguez-Emmenegger C ( Institute of Macromolecular Chemistry AS CR v.v.i., Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic); Dostálek J ( Biosensor Technologies, AIT-Austrian Institute of Technology GmbH, Muthgasse 11, 1190 Vienna, Austria. Electronic address: jakub.dostalek@ait.ac.at.) |
| Abstract | A plasmonic biosensor for rapid detection of protein biomarkers in complex media is reported. Clinical serum samples were analyzed by using a novel biointerface architecture based on poly[(N-(2-hydroxypropyl) methacrylamide)-co-(carboxybetaine methacrylamide)] brushes functionalized with bioreceptors. This biointerface provided an excellent resistance to fouling even after the functionalization and allowed for the first time the direct detection of antibodies against hepatitis B surface antigen (anti-HBs) in clinical serum samples using surface plasmon resonance (SPR). The fabricated SPR biosensor allowed discrimination of anti-HBs positive and negative clinical samples in 10min. Results are validated by enzyme-linked immunoassays of the sera in a certified laboratory. The sensor could be regenerated by simple treatment with glycine buffer. |
| ISSN | 09565663 |
| Journal | Biosensors and Bioelectronics |
| Volume Number | 85 |
| e-ISSN | 18734235 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-11-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nanoscience and Nanotechnology Medicine Biophysics Biomedical Engineering Biotechnology Electrochemistry |
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