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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Liu, Xiao-Bo Gu, Qiu-Ya Yu, Xiao-Bin |
| Description | Country affiliation: China Author Affiliation: Liu XB ( The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi, 214122 Jiangsu, China.) |
| Abstract | To improve butanol tolerance and production in Clostridium acetobutylicum, a novel approach was developed in this study, which was called artificial simulation of bio-evolution (ASBE) based on the evolutionary dynamics and natural selection. Through repetitive evolutionary domestications, a butanol-tolerant strain C. acetobutylicum T64 was obtained, which could withstand 4% (v/v) (compared to 2% of the wild-type) butanol and was accompanied by the increase of butanol production from 12.2g/L to 15.3g/L using corn meal as substrate. Fermentation was also carried out to investigate the relationship between butanol tolerance and ABE production, suggesting that enhancing butanol tolerance could increase butanol production but unlikely improve total ABE production. These results also indicated that the ASBE would be an available and feasible method used in biotechnology for enhancement of butanol tolerance and production. |
| ISSN | 09608524 |
| Volume Number | 130 |
| e-ISSN | 18732976 |
| Journal | Bioresource Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-02-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Biological Evolution Butanols Toxicity Clostridium Acetobutylicum Drug Effects Genetics Selection, Genetic Biofuels Cell Proliferation Cytology Kinetics Journal Article Research Support, Non-u.s. Gov't Discipline Bioresource |
| Content Type | Text |
| Resource Type | Article |
| Subject | Waste Management and Disposal Medicine Environmental Engineering Renewable Energy, Sustainability and the Environment Bioengineering |
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