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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Tong, Peng Sun, Wujin Wu, Jinglan Cheng, Jian He, Ying Ying, Hanjie Li, Nan Ji, Wenyan Chen, Xiaochun Chen, Yong Ouyang, Pingkai Xie, Jingjing |
| Description | Country affiliation: China Author Affiliation: Sun W ( State Key Laboratory of Materials-Oriented Chemical Engineering, National Engineering Technique Research Center for Biotechnology, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing, China.) |
| Abstract | Synthesis of N-acetylneuraminic acid (Neu5Ac) from N-acetylglucosamine (GlcNAc) and pyruvate was carried out by constructing and expressing a polycistronic plasmid encoding an N-acetylglucosamine 2-epimerase (AGE) gene and an N-acetylneuraminic acid lyase (Nal) gene simultaneously. Nal from Escherichia coli K12 and AGEs from Synechocystis sp. PCC 6803 (snAGE) and Anabaena sp. CH1 (anAGE) were used. And four polycistronic plasmids were constructed in which the positions of AGE gene differed with respect to Nal gene. Among these plasmids, pET-28a-Nal-anAGE with anAGE gene located next to Nal gene caused the production of the highest amount of Neu5Ac, generating 61.3g/L in 60h by whole-cell catalysis without the addition of ATP as AGE activator. And pET-28a-Nal-anAGE lowered anAGE's expression level, allowing it to fold properly. Thus, an inclusion-body-free E. coli strain capable of producing Neu5Ac by whole-cell catalysis with high yield and low cost was constructed in the present study. |
| ISSN | 09608524 |
| Volume Number | 130 |
| e-ISSN | 18732976 |
| Journal | Bioresource Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-02-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Acetylglucosamine Metabolism N-acetylneuraminic Acid Biosynthesis Oxo-acid-lyases Genetics Plasmids Anabaena Enzymology Carbohydrate Epimerases Catalysis Escherichia Coli Chemical Synthesis Synechocystis Journal Article Research Support, Non-u.s. Gov't Discipline Bioresource |
| Content Type | Text |
| Resource Type | Article |
| Subject | Waste Management and Disposal Medicine Environmental Engineering Renewable Energy, Sustainability and the Environment Bioengineering |
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