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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Wang, Damao Kim, Kyoung Heon Yun, Eun Ju Kim, Hee Taek Chung, Jae Hyuk Choi, In-Geol |
| Description | Author Affiliation: Kim HT ( School of Life Sciences and Biotechnology, Korea University, Seoul 136-713, Republic of Korea.) |
| Abstract | To obtain fermentable sugar from agarose, pretreatment of agarose by using acetic acid was conducted for short durations (10-30 min) at low acid concentrations (1-5% (w/v)) and high temperatures (110-130 °C). On testing the pretreated agarose by using an endo-ß-agarase I (DagA), an exo-ß-agarase II (Aga50D), and neoagarobiose hydrolase (NABH), we observed that the addition of the endo-type agarase did not increase the sugar yield. Use of the crude enzyme of Vibrio sp. EJY3 in combination with Aga50D and NABH including acetic acid pretreatment resulted in a 1.3-fold increase in the final reducing sugar yield (62.8% of theoretical maximum based on galactose and 3,6-anhydrogalactose in the initial agarose), compared to those obtained using Aga50D and NABH only after acetic acid pretreatment. The simultaneous saccharification and fermentation of pretreated agarose yielded ethanol of 37.1% theoretical maximum yield from galactose contained in the pretreated agarose. |
| ISSN | 09608524 |
| Volume Number | 136 |
| e-ISSN | 18732976 |
| Journal | Bioresource Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-05-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Acetic Acid Pharmacology Carbohydrates Biosynthesis Ethanol Metabolism Glycoside Hydrolases Hot Temperature Seaweed Sepharose Biomass Carbohydrate Metabolism Drug Effects Chromatography, Thin Layer Fermentation Furaldehyde Analogs & Derivatives Hydrolysis Vibrio Enzymology Journal Article Research Support, Non-u.s. Gov't Discipline Bioresource |
| Content Type | Text |
| Resource Type | Article |
| Subject | Waste Management and Disposal Medicine Environmental Engineering Renewable Energy, Sustainability and the Environment Bioengineering |
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