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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ramos, Luiz Pereira Silveira, Marcos Henrique Luciano Aguiar, Rodrigo Souza Siika-aho, Matti |
| Description | Country affiliation: Finland Author Affiliation: Silveira MH ( Research Center in Applied Chemistry (CEPESQ), Department of Chemistry, Federal University of Paraná, P.O. Box 19081, Curitiba, Paraná 81531-970, Brazil) |
| Abstract | The activity profile of a 1:0.30 mixture of Celluclast 1.5L FG and Novozym 188 (Novozymes) was investigated using Whatman #1 filter paper (W1FP) as a single substrate for hydrolysis. The procedure was based on the ability of the enzymes to release total (RS(Tot)), insoluble (RS(Insol)) and soluble (RS(Sol)) reducing sugars from W1FP. RS(Insol) was used to estimate endoglucanase (EnG) activity whereas exoglucanases (ExG) were assessed by measuring RSSol in the presence of δ-gluconolactone. Finally, the ß-glucosidase (ßG) activity was derived from the difference between RS(Sol) measurements in the presence and absence of δ-gluconolactone. When this analytical procedure was applied to W1FP using 9.64 mg mL(-1) of the enzyme mixture, the relative contributions of EnG, ExG and ßG to the total cellulase activity were 63.28%, 12.02% and 24.70%, respectively. Also, this ratio changed with changes in the enzyme loading, giving a new insight into the synergy that exists among the enzymes. |
| ISSN | 09608524 |
| Volume Number | 151 |
| e-ISSN | 18732976 |
| Journal | Bioresource Technology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-01-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Carbohydrate Metabolism Cellulases Metabolism Cellulose Beta-glucosidase Hydrolysis Oxidation-reduction Substrate Specificity Journal Article Research Support, Non-u.s. Gov't Discipline Bioresource |
| Content Type | Text |
| Resource Type | Article |
| Subject | Waste Management and Disposal Medicine Environmental Engineering Renewable Energy, Sustainability and the Environment Bioengineering |
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