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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Hannink, M. Donoghue, D. J. |
| Description | Author Affiliation: Hannink M ( Department of Chemistry, University of California, San Diego, La Jolla 92093.) |
| Abstract | Autocrine expression of a growth factor and its receptor in the same cell raises the possibility of an intracellular receptor-ligand interaction within the cell, in addition to a receptor-ligand interaction at the cell surface. We have constructed a NIH3T3 cell line which contains the v-sis gene under the inducible control of the Drosophila melanogaster hsp70 promoter. Expression of both v-sis RNA and protein is rapidly induced by a short period of heat-shock. We have analyzed the cellular site of interaction between the v-sis protein and the platelet-derived growth factor receptor in these cells. Autophosphorylation of the PDGF receptor and induction of the c-fos gene were found to occur at 45 and 50 min, respectively, after heat- induced synthesis of the v-sis protein. Monensin treatment of the heat- induced cells prevented autophosphorylation of the mature PDGF receptor and also prevented subsequent induction of c-fos. Autophosphorylation of the PDGF receptor and c-fos induction were also prevented by the addition of suramin to the medium. These results demonstrate that autocrine stimulation, as monitored by c-fos induction and by PDGF receptor autophosphorylation, requires an interaction between the v-sis protein and the PDGF receptor that occurs at the cell surface, rather than an intracellular location. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 1 |
| Volume Number | 107 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1988-07-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Cell Membrane Metabolism Gene Expression Regulation Receptors, Cell Surface Retroviridae Proteins Genetics Animals Cell Line Cloning, Molecular Drosophila Melanogaster Hot Temperature Kinetics Ligands Monensin Pharmacology Nucleic Acid Hybridization Oncogene Proteins V-sis Phosphorylation Promoter Regions, Genetic Proto-Oncogene Proteins Proto-Oncogene Proteins C-fos RNA Receptors, Platelet-Derived Growth Factor Suramin Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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