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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Wileman, T. Terhorst, C. Carson, G. R. Concino, M. Ahmed, A. |
| Description | Author Affiliation: Wileman T ( Laboratory of Molecular Immunology, Dana Farber Cancer Institute, Boston, Massachusetts 02115.) |
| Abstract | The T cell receptor for antigen (TCR) is composed of six different transmembrane proteins. T cells carefully control the intracellular transport of the receptor and allow only complete receptors to reach the plasma membrane. In an attempt to understand how T cells regulate this process, we used c-DNA transfection and subunit-specific antibodies to follow the intracellular transport of five subunits (alpha beta gamma delta epsilon) of the receptor. In particular, we assessed the intracellular stability of each chain. Our results showed that the chains were markedly different in their susceptibility to intracellular degradation. TCR alpha and beta and CD3 delta were degraded rapidly, whereas CD3 gamma and epsilon were stable. An analysis of the N-linked oligosaccharides of the glycoprotein subunits suggested that the chains were unable to reach the medial Golgi during the metabolic chase. This was supported by immunofluorescence micrographs that showed both the stable CD3 gamma and unstable CD3 delta chain localized in the endoplasmic reticulum. To study the effects of subunit associations on intracellular transport we used cotransfection to reconstitute precise combinations of subunits. Associations between stable and unstable subunits expressed in the same cell led to the formation of stable complexes. These complexes were retained in or close to the endoplasmic reticulum. The results suggested that the intracellular transport of the T cell receptor could be regulated by two mechanisms. The TCR alpha and beta and CD3 delta subunits were degraded rapidly and as a consequence failed to reach the plasma membrane. CD3 gamma or epsilon were stable but were retained inside the cell. The results also demonstrated that there was an interplay between the two pathways such that the CD3 gamma and epsilon subunits were able to protect labile chains from rapid intracellular degradation. In this way, they could seed subunit assembly in or close to the endoplasmic reticulum and allow a stable receptor to form before its transport to the plasma membrane. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 4 |
| Volume Number | 110 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1990-04-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Antigens, CD Immunology Antigens, Differentiation, T-Lymphocyte Receptors, Antigen, T-Cell Metabolism T-Lymphocytes Acetylglucosaminidase Animals Antigens, CD3 Genetics Isolation & Purification Cell Line Fluorescent Antibody Technique Genetic Vectors Golgi Apparatus Kinetics Lasers Macromolecular Substances Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase Biosynthesis Transfection Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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