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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Feeney, R. J. Zieve, G. W. |
| Description | Author Affiliation: Feeney RJ ( Department of Anatomical Sciences, State University of New York, Stony Brook 11794.) |
| Abstract | The snRNP particles include a set of common core snRNP proteins and snRNP specific proteins. In rodent cells the common core proteins are the B, D, D', E, F and G proteins in a suggested stoichiometry of B2D'2D2EFG. The additional U1- and U2-specific proteins are the 70-kD, A and C proteins and the A' and B" proteins, respectively. Previous cell fractionation and kinetic analysis demonstrated the snRNP core proteins are stored in the cytoplasm in large partially assembled snRNA- free intermediates that assemble with newly synthesized snRNAs during their transient appearance in the cytoplasm (Sauterer, R. A., R. J. Feeney, and G. W. Zieve. 1988. Exp. Cell Res. 176:344-359). This report investigates the assembly and intracellular distribution of the U1 and U2 snRNP-specific proteins. Cell enucleation and aqueous cell fractionation are used to prepare nuclear and cytoplasmic fractions and the U1- and U2-specific proteins are identified by isotopic labeling and immunoprecipitation or by immunoblotting with specific autoimmune antisera. The A, C, and A' proteins are found both assembled into mature nuclear snRNP particles and in unassembled pools in the nucleus that exchange with the assembled snRNP particles. The unassembled proteins leak from isolated nuclei prepared by detergent extraction. The unassembled A' protein sediments at 4S-6S in structures that may be multimers. The 70-kD and B" proteins are fully assembled with snRNP particles which do not leak from isolated nuclei. The kinetic studies suggest that the B" protein assembles with the U2 particle in the cytoplasm before it enters the nucleus. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 4 |
| Volume Number | 110 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1990-04-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Cell Nucleus Metabolism Ribonucleoproteins Animals Cytoplasm Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel HeLa Cells Immune Sera Immunoblotting L Cells (Cell Line) Mice Models, Biological Molecular Weight Isolation & Purification Ribonucleoproteins, Small Nuclear Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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