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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Blanks, J. E. Vestweber, D. Lenter, M. C. Borges, E. Zöllner, O. Steegmaier, M. Zerwes, H. G. |
| Description | Author Affiliation: Zöllner O ( Institute of Cell Biology, ZMBE, University of Münster, Germany.) |
| Abstract | L-Selectin on neutrophils as well as inducible E- and P-selectin on endothelium are involved in the recruitment of neutrophils into inflamed tissue. Based on cell attachment assays, L-selectin was suggested to function as a carbohydrate presenting ligand for E- and P-selectin. However, previous affinity isolation experiments with an E-selectin-Ig fusion protein had failed to detect L-selectin among the isolated E-selectin ligands from mouse neutrophils. We show here that L-selectin from human neutrophils, in contrast to mouse neutrophils, can be affinity-isolated as a major ligand from total cell extracts using E-selectin-Ig as affinity probe. Binding of human L-selectin to E-selectin was direct, since purified L-selectin could be reprecipitated with E-selectin-Ig. Recognition of L-selectin was abolished by sialidase-treatment, required Ca2+, and was resistant to treatment with endoglycosidase F. Binding of L-selectin to a P-selectin-Ig fusion protein was not observed. In agreement with the biochemical data, the anti-L-selectin mAb DREG56 inhibited rolling of human neutrophils on immobilized E-selectin-Ig but not on P-selectin-Ig. No such inhibitory effect was seen with the anti-mouse L-selectin mAb MEL14 on mouse neutrophils. Rolling of E-selectin transfectants on purified and immobilized human L-selectin was inhibited by mAb DREG56. We conclude that L-selectin on human neutrophils is a major glycoprotein ligand among very few glycoproteins that can be isolated by an E-selectin affinity matrix. The clear difference between human and mouse L-selectin suggests that E-selectin-binding carbohydrate moieties are attached to different protein scaffolds in different species. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 3 |
| Volume Number | 136 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1997-02-10 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | E-Selectin Metabolism L-Selectin Neutrophils Animals Antibodies, Monoclonal Bone Marrow Cells CHO Cells Cricetinae Genetics Immunoglobulin G Mannosyl-Glycoprotein Endo-beta-N-Acetylglucosaminidase Mice Recombinant Fusion Proteins Sialic Acids Research Support, Non-U.S. Gov't Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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