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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Lenter, M. Isenmann, S. Vestweber, D. Levinovitz, A. |
| Description | Author Affiliation: Lenter M ( Hans Spemann Laboratory, Max Planck Institute for Immunology, Freiburg, Germany.) |
| Abstract | E- and P-selectin are inducible cell adhesion molecules on endothelial cells, which function as Ca(2+)-dependent lectins and mediate the binding of neutrophils and monocytes. We have recently identified a 150- kD glycoprotein ligand for E-selectin on mouse myeloid cells, using a recombinant antibody-like form of mouse E-selectin. Here, we report that this ligand does not bind to an analogous P-selectin fusion protein. Instead, the chimeric P-selectin-IgG protein recognizes a 160- kD glycoprotein on the mouse neutrophil progenitor 32D cl 3, on mature mouse neutrophils and on human HL60 cells. The binding is Ca(2+)- dependent and requires the presence of sialic acid on the ligand. This P-selectin-ligand is not recognized by E-selectin. Removal of N-linked carbohydrate side chains from the 150-kD and the 160-kD monospecific selectin ligands abolishes the binding of both ligands to the respective selectin. Treatment of HL60 cells with Peptide: N- glycosidase F inhibited cell binding to P- and E-selectin. In addition, glycoproteins of 230 and 130 kD were found on mature mouse neutrophils, which bound both to E- and P-selectin in a Ca(2+)-dependent fashion. The signals detected for these ligands were 15-20-fold weaker than those for the monospecific ligands. Both proteins were heavily sialylated and selectin-binding was blocked by removal of sialic acid, but not by removal of N-linked carbohydrates. Our data reveal that E- and P-selectin recognize two categories of glycoprotein ligands: one type requires N-linked carbohydrates for binding and is monospecific for each of the two selectins and the other type binds independent of N- linked carbohydrates and is common for both endothelial selectins. |
| ISSN | 00219525 |
| e-ISSN | 15408140 |
| Journal | The Journal of Cell Biology |
| Issue Number | 2 |
| Volume Number | 125 |
| Language | English |
| Publisher | Rockefeller University Press (United States) |
| Publisher Date | 1994-04-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Cell Adhesion Molecules Metabolism Glycoproteins Leukocytes, Mononuclear Platelet Membrane Glycoproteins Amidohydrolases Animals Carbohydrate Metabolism Cell Adhesion Immunology Cell Line E-Selectin Immunoglobulin G Leukemia, Myeloid Ligands Metalloendopeptidases Mice Neutrophils P-Selectin Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase Sialic Acids Tumor Cells, Cultured Research Support, Non-U.S. Gov't Cell Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Medicine |
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