NDLI: Actin S-nitrosylation inhibits neutrophil beta2 integrin function.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bhopale, Veena M. Milovanova, Tatyana N. Thom, Stephen R. Mancini, D. Joshua
Description	Author Affiliation: Thom SR ( Institute for Environmental Medicine, Department of Emergency Medicine, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania 19104-6068, USA.)
Abstract	The focus of this work was to elucidate the mechanism for inhibition of neutrophil beta(2) integrin adhesion molecules by hyperoxia. Results demonstrate that exposure to high oxygen partial pressures increases synthesis of reactive species derived from type 2 nitric-oxide synthase and myeloperoxidase, leading to excessive S-nitrosylation of beta-actin and possibly profilin. Hyperoxia causes S-nitrosylation of the four cysteine moieties closest to the carboxyl-terminal end of actin, which results in formation of short actin filaments. This alters actin polymerization, network formation, and intracellular distribution, as well as inhibits beta(2) integrin clustering. If neutrophils are exposed to ultraviolet light to reverse S-nitrosylation, or are incubated with N-formyl-methionyl-leucine-phenylalanine to trigger 'inside-out' activation, the effects of hyperoxia are reversed. We conclude that cytoskeletal changes triggered by hyperoxia inhibit beta(2) integrin-dependent neutrophil adhesion.
ISSN	00219258
e-ISSN	1083351X
Journal	Journal of Biological Chemistry
Issue Number	16
Volume Number	283
Language	English
Publisher	American Society for Biochemistry and Molecular Biology (United States)
Publisher Date	2008-04-18
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Actins Physiology Antigens, CD18 Metabolism Neutrophils Amino Acid Sequence Animals Biosynthesis Cell Adhesion Hyperoxia Mice Mice, Knockout Molecular Sequence Data Oxygen Peroxidase Profilins Research Support, U.S. Gov't, Non-P.H.S. Biochemistry Molecular Biology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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