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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Severinov, Konstantin Kuznedelov, Konstantin Kulbachinskiy, Andrey Barinova, Nataliya |
| Description | Author Affiliation: Barinova N ( Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 123182, Russia.) |
| Abstract | We recently described a novel basal bacterial promoter element that is located downstream of the -10 consensus promoter element and is recognized by region 1.2 of the sigma subunit of RNA polymerase (RNAP). In the case of Thermus aquaticus RNAP, this element has a consensus sequence GGGA and allows transcription initiation in the absence of the -35 element. In contrast, the Escherichia coli RNAP is unable to initiate transcription from GGGA-containing promoters that lack the -35 element. In the present study, we demonstrate that sigma subunits from both E. coli and T. aquaticus specifically recognize the GGGA element and that the observed species specificity of recognition of GGGA-containing promoters is determined by the RNAP core enzyme. We further demonstrate that transcription initiation by T. aquaticus RNAP on GGGA-containing promoters in the absence of the -35 element requires sigma region 4 and C-terminal domains of the alpha subunits, which interact with upstream promoter DNA. When in the context of promoters containing the -35 element, the GGGA element is recognized by holoenzyme RNAPs from both E. coli and T. aquaticus and increases stability of promoter complexes formed on these promoters. Thus, GGGA is a bona fide basal promoter element that can function in various bacteria and, depending on the properties of the RNAP core enzyme and the presence of additional promoter elements, determine species-specific differences in promoter recognition. |
| ISSN | 00219258 |
| e-ISSN | 1083351X |
| Journal | Journal of Biological Chemistry |
| Issue Number | 33 |
| Volume Number | 283 |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology (United States) |
| Publisher Date | 2008-08-15 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | DNA-Directed RNA Polymerases Metabolism Escherichia Coli Genetics Promoter Regions, Genetic Thermus Transcription, Genetic Bacterial Proteins Enzymology Escherichia Coli Proteins Oligodeoxyribonucleotides Chemistry Oligoribonucleotides Substrate Specificity Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Biochemistry Molecular Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology |
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